gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water.

A Gram-negative, heterotrophic, marine bacterium, designated strain TT1, was isolated from seawater collected from the shallow coastal region of Anping Harbour, Tainan, Taiwan. Cells grown in broth cultures were straight rods that were motile by means of a single polar flagellum. The isolate required NaCl for growth and exhibited optimal growth at 30-35 °C, 2-4 % NaCl and pH 7-8. Strain TT1 grew aerobically and was not capable of anaerobic growth by fermentation of glucose or other carbohydrates. Q-10 was the sole major isoprenoid quinone. Cellular fatty acids were dominated by Cω7 (47.5 %), C 2-OH (16.3 %) and Cω8 cyclo (10.6 %). The DNA G+C content was 56.4 mol%. Phylogeny based on 16S rRNA gene sequences showed that strain TT1 formed a distinct genus-level lineage in the family of the class and exhibited the highest sequence similarity with species of the genera (89.9-90.0 % 16S rRNA gene sequence similarity), (89.4-89.9 %) and (89.1-89.7 %). Strain TT1 could be distinguished from species of these phylogenetically closest genera based on differences in DNA G+C contents (56.4 mol% vs 60.0-68.0 mol%), fatty acid profiles and some physiological characteristics. On the basis of the polyphasic taxonomic data from this study, strain TT1 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is TT1 ( = JCM 18659 = BCRC 80493).