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Lineage-Specific Profiling Delineates the Emergence and Progression of Naive Pluripotency in Mammalian Embryogenesis. | LitMetric

Lineage-Specific Profiling Delineates the Emergence and Progression of Naive Pluripotency in Mammalian Embryogenesis.

Dev Cell

Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK; European Bioinformatics Institute, European Molecular Biology Laboratory, Wellcome Trust Genome Campus, Cambridge CB10 1SD, UK; Genome Biology and Developmental Biology Units, European Molecular Biology Laboratory, Meyerhofstraße 1, 69117 Heidelberg, Germany. Electronic address:

Published: November 2015

AI Article Synopsis

  • The study investigates the gene expression dynamics in mouse embryos from the eight-cell stage to after implantation, utilizing lineage-specific RNA sequencing to analyze various embryonic cell types.
  • Researchers identify key expression patterns that characterize naive pluripotency and how it changes over time, observing similarities in transcriptional networks across species.
  • The findings also suggest that while the core transcriptional machinery for naive pluripotency is conserved, different species like marmosets and rodents may rely on distinct signaling pathways for early developmental processes.

Article Abstract

Naive pluripotency is manifest in the preimplantation mammalian embryo. Here we determine transcriptome dynamics of mouse development from the eight-cell stage to postimplantation using lineage-specific RNA sequencing. This method combines high sensitivity and reporter-based fate assignment to acquire the full spectrum of gene expression from discrete embryonic cell types. We define expression modules indicative of developmental state and temporal regulatory patterns marking the establishment and dissolution of naive pluripotency in vivo. Analysis of embryonic stem cells and diapaused embryos reveals near-complete conservation of the core transcriptional circuitry operative in the preimplantation epiblast. Comparison to inner cell masses of marmoset primate blastocysts identifies a similar complement of pluripotency factors but use of alternative signaling pathways. Embryo culture experiments further indicate that marmoset embryos utilize WNT signaling during early lineage segregation, unlike rodents. These findings support a conserved transcription factor foundation for naive pluripotency while revealing species-specific regulatory features of lineage segregation.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643313PMC
http://dx.doi.org/10.1016/j.devcel.2015.10.011DOI Listing

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