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Molecular and immunological methods to detect rotavirus in formalin-fixed tissue.
J Virol Methods
September 2002
Division of Viral and Rickettsial Disease, Centers for Disease Control and Prevention, National Center for Infectious Diseases, Infectious Disease Pathology Activity, 1600 Clifton Road, NE, Mailstop G30, Atlanta, GA 30333, USA.
In 1999, a tetravalent rhesus-based rotavirus vaccine was withdrawn from the market after reports of intussusception cases among vaccinated infants. Methods to detect rotavirus in formalin-fixed pathology specimens from such patients will be important in examining the possible associations between the vaccine and intussusception, in investigating fatalities caused by natural rotavirus infection, and in furthering our understanding of the pathogenesis of rotavirus disease. Three different methods, reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry (IHC), and in situ hybridization (ISH), were developed to detect rotavirus in infected cell lines that were fixed in formalin and embedded in paraffin.
View Article and Find Full Text PDFPurification and characterization of nuclear alkaline phospholipase A2 in rat ascites hepatoma cells.
FEBS Lett
September 1996
Second Department of Internal Medicine, Nagoya University School of Medicine, Japan.
The alkaline phospholipase A2 (PLA2) was purified from nuclei of rat ascites hepatoma cells (AH7974) by column chromatography with a Sephacryl S-300 column and an immunoadsorbent using anti-group II PLA2 monoclonal antibody. From these two columns, the alkaline PLA2 was eluted in parallel with a 17-kDa protein which is reactive to another anti-group II PLA2 polyclonal antibody. Approximately 80% of nuclear PLA2 was inhibited by this antibody.
View Article and Find Full Text PDFMonoclonal antibodies to group II Dermatophagoides spp. allergens: murine immune response, epitope analysis, and development of a two-site ELISA.
J Allergy Clin Immunol
September 1994
UVA Asthma and Allergic Diseases Center, Charlottesville 22908.
Background: Group II allergens are a major cause of sensitization in patients allergic to mites. To facilitate the antigenic analysis of group II allergens and to develop improved methods of allergen detection, we compared IgG anti-group II antibody responses in inbred mouse strains and raised a panel of monoclonal antibodies (mAbs).
Methods: IgE antibody responses were compared by antigen-binding radioimmunoassay.
A survey of G6 and G10 serotypes of group A bovine rotaviruses from diarrheic beef and dairy calves using monoclonal antibodies in ELISA.
J Vet Diagn Invest
April 1994
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
Group A bovine rotaviruses (BRV) have been identified worldwide as a major cause of diarrhea in the young of many species, including humans. Group A rotaviruses are classified into serotypes on the basis of the outer capsid proteins, VP7 (G types) and VP4 (P types). To date, there are 14 G types of group A rotaviruses, with G1, G6, G8, and G10 described for BRV isolates.
View Article and Find Full Text PDFPolyclonal anti-group B Streptococcus latex antigen detection test.
Pediatr Infect Dis J
April 1989
Department of Laboratories, St. Christopher's Hospital for Children, Philadelphia, PA 19133.
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