We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mimet.2015.11.002 | DOI Listing |
Publication Analysis
Top Keywords
quellung reaction
8
capsular typing
4
typing streptococcus
4
streptococcus pneumoniae
4
pneumoniae isolated
4
isolated algerian
4
algerian hospital
4
hospital multiplex
4
multiplex pcr-based
4
pcr-based scheme
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!