AI Article Synopsis
- A new nanosensor has been developed to detect tyrosine using a technique called photoinduced electron-transfer (PET) involving NaYF4:Yb, Tm upconversion nanoparticles and melanin-like polymers.
- The melanin-like films are created by oxidizing tyrosine with the enzyme tyrosinase and deposited on the nanoparticles, leading to a decrease in their fluorescence.
- The sensor demonstrated a linear response to tyrosine concentrations from 0.8 to 100 μM, with a detection limit of 1.1 μM, and proved effective in detecting tyrosine levels in human serum, showcasing good sensitivity and stability.
Article Abstract
This paper reports a novel nanosensor for tyrosine based on photoinduced electron-transfer (PET) between NaYF4:Yb, Tm upconversion nanoparticles (UCNPs) and melanin-like polymers. Melanin-like films were obtained from catalytic oxidation of tyrosine by tyrosinase, and deposited on the surface of UCNPs, and then quenched the fluorescence of UCNPs. Under the optimized conditions, the fluorescence quenching of UCNPs showed a good linear response to tyrosine concentration in the range of 0.8-100 μΜ with a detection limit of 1.1 μΜ. Meanwhile, it showed good sensitivity, stability and has been successfully applied to the detection of tyrosine in human serum.
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| http://dx.doi.org/10.1016/j.bios.2015.10.084 | DOI Listing |
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