Plasma progesterone (P) and testosterone (T) were determined by enzyme immunology with commercially available kits. Two kits (Serono and BioMérieux) were studied for P determination and one kit (Serono) for that of the T. The results obtained by either enzyme immunoassay were compared reliable and specific radioimmunoassays. The BioMérieux kit was shown to be more appropriate than the Serono kit for plasma P assay except in patients after oral administration of micronized progesterone because of the interference of the high levels of P metabolites. Concerning T, the Serono kit appears to yield generally adequate results in male adults but neither in men treated with dihydrotestosterone nor in females.
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