AI Article Synopsis
- This study investigated how human mesenchymal stem cells (hMSCs) grow and differentiate when cultured on hydroxyapatite (HA) and mineralized collagen (MC).
- Findings indicated that mineralized collagen (MC) enhances the proliferation and osteogenic differentiation of hMSCs, as measured by alkaline phosphatase levels.
- Microarray analysis revealed that MC increases the expression of key osteogenesis-related genes (like BMP-2, COL1A1, and CTSK) and promotes pathways related to bone development.
Article Abstract
In this study, human mesenchymal stem cells (hMSCs) were cultured on the hydroxyapatite (HA) and mineralized collagen (MC), and their proliferation, adhesion, and differentiation, especially the molecular mechanisms on gene level, were investigated. Proliferation and morphological responses of hMSCs and their osteogenic differentiation were detected by quantitative detection of alkaline phosphatase. Gene expression profilings were examined by microarrays, and the gene expression data were studied through gene ontology terms and pathway analyses. The results showed that MC promoted cell proliferation and osteogenic differentiation of hMSCs. Microarray analysis showed that MC was conducive to express osteogenesis-related genes, such as BMP-2, COL1A1, and CTSK, and stimulate osteogenic differentiation, such as osteoblast differentiation pathway and skeletal system development pathway.
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| http://dx.doi.org/10.1089/ten.tea.2015.0237 | DOI Listing |
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