AI Article Synopsis
- * One example is a red seaweed called Delisea pulchra that can be affected by a bacteria called Nautella italica R11, which makes it turn white or "bleach."
- * Scientists found a special gene called varR in the bacteria that helps it attach and cause disease on the seaweed, and without this gene, the bacteria can't make the seaweed sick.
Article Abstract
There is increasing evidence to suggest that macroalgae (seaweeds) are susceptible to infectious disease. However, to date, little is known about the mechanisms that facilitate the colonization and virulence of microbial seaweed pathogens. One well-described example of a seaweed disease is the bleaching of the red alga Delisea pulchra, which can be caused by the bacterium Nautella italica R11, a member of the Roseobacter clade. This pathogen contains a unique luxR-type gene, varR, which we hypothesize controls its colonization and virulence. We show here that a varR knock-out strain is deficient in its ability to cause disease in D. pulchra and is defective in biofilm formation and attachment to a common algal polysaccharide. Moreover complementation of the varR gene in trans can restore these functions to the wild type levels. Proteomic analysis of bacterial cells in planktonic and biofilm growth highlight the potential importance of nitrogen scavenging, mobilization of energy reserves, and stress resistance in the biofilm lifestyle of N. italica R11. Moreover, we show that VarR regulates the expression of a specific subset of biofilm-associated proteins. Taken together these data suggest that VarR controls colonization and persistence of N. italica R11 on the surface of a macroalgal host and that it is an important regulator of virulence.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4602140 | PMC |
| http://dx.doi.org/10.3389/fmicb.2015.01130 | DOI Listing |
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Article Synopsis
- * One example is a red seaweed called Delisea pulchra that can be affected by a bacteria called Nautella italica R11, which makes it turn white or "bleach."
- * Scientists found a special gene called varR in the bacteria that helps it attach and cause disease on the seaweed, and without this gene, the bacteria can't make the seaweed sick.
A glutathione peroxidase (GpoA) plays a role in the pathogenicity of Nautella italica strain R11 towards the red alga Delisea pulchra.
FEMS Microbiol Ecol
April 2015
CMB, University of New South Wales, Sydney, NSW 2052, Australia
Oxidative bursts are a common mechanism used by higher organisms to defend themselves against bacterial attacks, but some pathogenic bacteria have evolved resistance mechanisms to counteract this. The role of oxidative stress resistance as a virulence trait in macroalgal disease is however unknown. Here, we demonstrate that the gene gpoA, encoding for a glutathione peroxidase, is important for the oxidative stress response of the macroalgal pathogen Nautella italica R11.
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