Dynamics of yeast immobilized-cell fluidized-bed bioreactors systems in ethanol fermentation from lactose-hydrolyzed whey and whey permeate.

Bioprocess Biosyst Eng

Biotechnology and Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.

Published: January 2016

AI Article Synopsis

  • The study explored ethanol production using lactose-hydrolyzed whey and its permeate, testing different strains of yeast in bioreactors.
  • Co-cultures of Saccharomyces cerevisiae and Kluyveromyces marxianus significantly outperformed single cultures in ethanol productivity, but K. marxianus alone yielded the highest results in both types of media.
  • Ethanol production was maximized with concentrated whey permeate, achieving high yields and improvements in productivity with repeated batches.

Article Abstract

We studied the dynamics of ethanol production on lactose-hydrolyzed whey (LHW) and lactose-hydrolyzed whey permeate (LHWP) in batch fluidized-bed bioreactors using single and co-cultures of immobilized cells of industrial strains of Saccharomyces cerevisiae and non-industrial strains of Kluyveromyces marxianus. Although the co-culture of S. cerevisiae CAT-1 and K. marxianus CCT 4086 produced two- to fourfold the ethanol productivity of single cultures of S. cerevisiae, the single cultures of the K. marxianus CCT 4086 produced the best results in both media (Y EtOH/S = 0.47-0.49 g g(-1) and Q P = 1.39-1.68 g L(-1) h(-1), in LHW and LHWP, respectively). Ethanol production on concentrated LHWP (180 g L(-1)) reached 79.1 g L(-1), with yields of 0.46 g g(-1) for K. marxianus CCT 4086 cultures. Repeated batches of fluidized-bed bioreactor on concentrated LHWP led to increased ethanol productivity, reaching 2.8 g L(-1) h(-1).

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http://dx.doi.org/10.1007/s00449-015-1498-0DOI Listing

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