Multivalent self-assembly of trifunctional aromatic propellers and ssDNA results in the formation of chiral supramolecular assemblies that can be used for the detection of small fragments of ssDNA with different lengths and compositions.
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http://dx.doi.org/10.1039/c5cc08283h | DOI Listing |
Proc Natl Acad Sci U S A
December 2024
Biocenter, Johannes Gutenberg University Mainz, Mainz 55128, Germany.
Intrinsically disordered proteins (IDPs) adopt ensembles of rapidly fluctuating heterogeneous conformations, influencing their binding capabilities and supramolecular transitions. The primary conformational descriptors for understanding IDP ensembles-the radius of gyration (), measured by small-angle X-ray scattering (SAXS), and the root mean square (rms) end-to-end distance (), probed by fluorescent resonance energy transfer (FRET)-are often reported to produce inconsistent results regarding IDP expansion as a function of denaturant concentration in the buffer. This ongoing debate surrounding the FRET-SAXS discrepancy raises questions about the overall reliability of either method for quantitatively studying IDP properties.
View Article and Find Full Text PDFNano Lett
December 2023
Joint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry, The First Hospital of Jilin University, Changchun, 130021, P. R. China.
Novel high-throughput protein detection technologies are critically needed for population-based large-scale SARS-CoV-2 antibody detection as well as for monitoring quality and duration of immunity against virus variants. Current protein microarray techniques rely heavily on labeled transduction methods that require sophisticated instruments and complex operations, limiting their clinical potential, particularly for point-of-care (POC) applications. Here, we developed a label-free and naked-eye readable microarray (NRM) based on a thickness-sensing plasmon ruler, enabling antibody profiling within 30 min.
View Article and Find Full Text PDFAdv Mater
January 2020
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130021, P. R. China.
Optical biosensors, especially those based on plasmonic structures, have emerged recently as a potential tool for disease diagnostics. Plasmonic biosensors have demonstrated impressive benefits for the label-free detection of trace biomarkers in human serum. However, widespread applications of these technologies are hindered because of their insufficient sensitivity, their relatively complex chemical immobilization processes, and the use of prism couplers.
View Article and Find Full Text PDFMethods Cell Biol
August 2017
University of California, Berkeley, CA, United States.
Much about septin function has been inferred from in vivo studies using mainly genetic methods, and much of what we know about septin organization has been obtained through examination of static structures in vitro primarily by electron microscopy. Deeper mechanistic insight requires real-time analysis of the dynamics of the assembly of septin-based structures and how other proteins associate with them. We describe here a Förster resonance energy transfer (FRET)-based approach for measuring in vitro the rate and extent of filament formation from septin complexes, binding of other proteins to septin structures, and the apparent affinities of these interactions.
View Article and Find Full Text PDFChem Commun (Camb)
January 2016
Adaptive Supramolecular Nanosystems Group, Institut Européen des Membranes, University of Montpellier/ENSCM/CNRS 5635, Pl. Eugène Bataillon, CC 047, 34095 Montpellier, Cedex 5, France.
Multivalent self-assembly of trifunctional aromatic propellers and ssDNA results in the formation of chiral supramolecular assemblies that can be used for the detection of small fragments of ssDNA with different lengths and compositions.
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