AI Article Synopsis
- The study aimed to assess the effectiveness of an enzyme immunoassay (EIA) for detecting antibodies to a specific antigen related to blastomycosis in dogs and compare it with other diagnostic tests.
- Researchers collected serum and urine samples from 70 dogs, including those with blastomycosis and healthy controls, testing them for antibodies and antigens associated with the disease.
- The EIA for antibodies showed a higher sensitivity (95%) compared to another test (65%) and demonstrated good specificity, making it a potentially valuable tool for differentiating blastomycosis from other conditions like histoplasmosis.
Article Abstract
Objective: To evaluate the sensitivity and specificity of an enzyme immunoassay (EIA) for antibodies to a recombinant Blastomyces adhesin-1 repeat antigen (rBAD-1) to aid in the diagnosis of blastomycosis in dogs and compare the findings with results from other tests used for this purpose.
Design: Prospective analytic study.
Sample: Serum and urine from 70 dogs with and without blastomycosis.
Procedures: Serum and urine samples were collected from dogs with blastomycosis (n = 21), histoplasmosis (8), or nonfungal pulmonary disease (21) and from healthy control dogs living in a blastomycosis-endemic area (20). Serum was tested for antibodies against Blastomyces dermatitidis with the rBAD-1 antibody EIA and an A-antigen antibody agar gel immunodiffusion (AGID) assay. Serum and urine were tested for B dermatitidis antigen with a quantitative EIA.
Results: Sensitivity of the quantitative antigen EIA was 100% in serum and urine samples from dogs with blastomycosis, with specificity of 95% in urine samples from dogs with nonfungal pulmonary disease and 100% in urine samples from healthy dogs. Sensitivity of the rBAD-1 antibody EIA (95%) was significantly greater than that of the A-antigen antibody AGID assay (65%). Specificity of the antibody EIA was 88% in dogs with histoplasmosis, 95% in healthy dogs, and 100% in dogs with nonfungal pulmonary disease.
Conclusions And Clinical Relevance: The rBAD-1 antibody EIA had greater sensitivity than the A-antigen antibody AGID assay in dogs with blastomycosis. This antibody EIA may assist in distinguishing histoplasmosis from blastomycosis. Further evaluation in a larger prospective study is needed to verify these results.
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| http://dx.doi.org/10.2460/javma.247.10.1133 | DOI Listing |
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