AI Article Synopsis
- A cross between 'Wanhongzhu' and 'Lapins' sweet cherry cultivars was created to develop a genetic mapping population for linkage map construction.
- Using the specific-locus amplified fragment (SLAF) sequencing technique, researchers discovered 701 SNPs and built a detailed map with 8 linkage groups covering 849.0 cM, maintaining marker collinearity with peach genomic data.
- The map identified that trunk diameter (TD) is influenced by 4 distinct loci across 3 linkage groups, highlighting its potential for pinpointing agronomically important traits and boosting sweet cherry breeding efforts.
Article Abstract
A cross between the sweet cherry (Prunus avium) cultivars 'Wanhongzhu' and 'Lapins' was performed to create a mapping population suitable for the construction of a linkage map. The specific-locus amplified fragment (SLAF) sequencing technique used as a single nucleotide polymorphism (SNP) discovery platform and generated 701 informative genotypic assays; these, along with 16 microsatellites (SSRs) and the incompatibility (S) gene, were used to build a map which comprised 8 linkage groups (LGs) and covered a genetic distance of 849.0 cM. The mean inter-marker distance was 1.18 cM and there were few gaps > 5 cM in length. Marker collinearity was maintained with the established peach genomic sequence. The map was used to show that trunk diameter (TD) is under the control of 4 loci, mapping to 3 different LGs. Different locus influenced TD at a varying stage of the tree's development. The high density 'W×L' genetic linkage map has the potential to enable high-resolution identification of QTLs of agronomically relevant traits, and accelerate sweet cherry breeding.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627659 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0141261 | PLOS |
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