Members of the Mycoplasma mycoides cluster' represent important livestock pathogens worldwide. Mycoplasma mycoides subsp. mycoides is the etiologic agent of contagious bovine pleuropneumonia (CBPP), which is still endemic in many parts of Africa. We report the genome sequences and annotation of two frequently used challenge strains of Mycoplasma mycoides subsp. mycoides, Afadé and B237. The information provided will enable downstream 'omics' applications such as proteomics, transcriptomics and reverse vaccinology approaches. Despite the absence of Mycoplasma pneumoniae like cyto-adhesion encoding genes, the two strains showed the presence of protrusions. This phenotype is likely encoded by another set of genes.
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http://dx.doi.org/10.1186/s40793-015-0067-0 | DOI Listing |
Heliyon
December 2024
Goat Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka, 1341, Bangladesh.
Background And Objective: Contagious caprine pleuropneumonia (CCPP) is a highly contagious mycoplasmal respiratory disease primarily affecting goats and sheep caused by subsp. (Mccp). So far, there is no available information on either the serological or molecular identification of Mccp in Bangladesh.
View Article and Find Full Text PDFVet Anim Sci
December 2024
Department of Veterinary Epidemiology and Public Health, College of Veterinary Medicine and Animal Sciences, University of Gondar, Ethiopia.
Methods Mol Biol
November 2024
Bioinformatics and Computational Biology Program, Iowa State University, Ames, IA, USA.
The JCVI-Syn3 organism is a minimal organism derived from Mycoplasma mycoides capri, which is capable of self-replication. While the ancestor has 863 genes, the synthetic progeny has only 473, with 434 of these coding for proteins. Despite initial efforts to understand all functions of the organism, a significant number of these protein-coding genes still have unknown functions, and subsequent studies have been only partially successful in elucidating their roles.
View Article and Find Full Text PDFMicrob Genom
November 2024
Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115201, Taiwan, ROC.
Genome-based analysis allows for large-scale classification of diverse bacteria and has been widely adopted for delineating species. Unfortunately, for higher taxonomic ranks such as genus, establishing a generally accepted approach based on genome analysis is challenging. While core-genome phylogenies depict the evolutionary relationships among species, determining the correspondence between clades and genera may not be straightforward.
View Article and Find Full Text PDFNat Commun
November 2024
Technische Universität Dresden, B CUBE Center for Molecular Bioengineering, Dresden, Germany.
All cells are encapsulated by a lipid membrane that facilitates their interactions with the environment. How cells manage diverse mixtures of lipids, which dictate membrane property and function, is experimentally challenging to address. Here, we present an approach to tune and minimize membrane lipid composition in the bacterium Mycoplasma mycoides and its derived 'minimal cell' (JCVI-Syn3A), revealing that a two-component lipidome can support life.
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