AI Article Synopsis
- A transcriptome study on Sulfolobus islandicus REY15A showed that the organism acquired CRISPR spacers from the virus STSV2 while growing in different types of media over six days.
- The acquisition of spacers led to a slowdown in host growth, changes in the activity of various CRISPR-Cas modules, and fluctuating viral copy numbers, with notable differences between the media types.
- Additionally, the study found that certain proteins crucial for cell cycle progression had reduced transcript levels, while others related to DNA management and transposases showed increased or stable expression, suggesting a complex regulatory network involving antisense RNAs and coordination between CRISPR-Cas system modules.
Article Abstract
A transcriptome study was performed on Sulfolobus islandicus REY15A actively undergoing CRISPR spacer acquisition from the crenarchaeal monocaudavirus STSV2 in rich and basal media over a 6 day period. Spacer acquisition preceded strong host growth retardation, altered transcriptional activity of four different CRISPR-Cas modules and changes in viral copy numbers, and with significant differences in the two media. Transcript levels of proteins involved in the cell cycle were reduced, whereas those of DNA replication, DNA repair, transcriptional regulation and some antitoxin-toxin pairs and transposases were unchanged or enhanced. Antisense RNAs were implicated in the transcriptional regulation of adaptation and interference modules of the type I-A CRISPR-Cas system, and evidence was found for the occurrence of functional co-ordination between the single CRISPR-Cas adaptation module and the functionally diverse interference modules.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/mmi.13263 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
CRISPR-Cas spacer acquisition is a rare event in human gut microbiome.
Cell Genom
December 2024
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA; Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address:
Host-parasite relationships drive the evolution of both parties. In microbe-phage dynamics, CRISPR functions as an adaptive defense mechanism, updating immunity via spacer acquisition. Here, we investigated these interactions within the human gut microbiome, uncovering low frequencies of spacer acquisition at an average rate of one spacer every ∼2.
View Article and Find Full Text PDFComprehensive Analysis of CRISPR-Cas Systems and Their Influence on Antibiotic Resistance in Strains.
Bioinform Biol Insights
December 2024
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
is a gram-negative bacterium that demonstrates a remarkable ability to acquire antibiotic resistance genes (ARGs). The role of the CRISPR-Cas system in influencing antibiotic resistance in is still under investigation. This study explores the distribution and impact of CRISPR-Cas systems on antibiotic resistance by analyzing 316 genomes.
View Article and Find Full Text PDFAdaptive immunity of type VI CRISPR-Cas systems associated with reverse transcriptase-Cas1 fusion proteins.
Nucleic Acids Res
December 2024
Department of Soil and Plant Microbiology, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Structure, Dynamics and Function of Bacterial Genomes, Grupo de Ecología Genética de la Rizosfera, C/Profesor Albareda 1, 18008 Granada, Spain.
Cas13-containing type VI CRISPR-Cas systems specifically target RNA; however, the mechanism of spacer acquisition remains unclear. We have previously reported the association of reverse transcriptase-Cas1 (RT-Cas1) fusion proteins with certain types of VI-A systems. Here, we show that RT-Cas1 fusion proteins are also recruited by type VI-B systems in bacteria from gut microbiomes, constituting a VI-B1 variant system that includes a CorA-encoding locus in addition to the CRISPR array and the RT-Cas1/Cas2 adaptation module.
View Article and Find Full Text PDFGenome mining approach reveals the CRISPR-Cas systems features and characteristics in strains.
Heliyon
November 2024
Department of Food Biotechnology, Branch for Northwest & West region, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Tabriz, Iran.
Article Synopsis
- * It found that 56% of the identified CRISPR-Cas systems belong to subtypes I-E/I-C, 23% to III-A/III-D, and 17% to II-A, with specific plasmids targeted by distinct systems.
- * The research concluded that strains with type-I CRISPR-Cas systems have a wider range of phage targets, highlighting the critical role of these systems in protecting against viral infections.
CRISPR-Cas systems confer adaptive immunity to their prokaryotic hosts through the process of adaptation, where sequences are captured from foreign nucleic acids and integrated as spacers in the CRISPR array, and thereby enable crRNA-guided interference against new threats. While the Cas1-2 integrase is critical for adaptation, it is absent from many CRISPR-Cas loci, rendering the mechanism of spacer acquisition unclear for these systems. Here we show that the RNA-targeting type VI-A CRISPR system of acquires spacers from DNA substrates through the action of a promiscuous Cas1-2 integrase encoded by a co-occurring type II-C system, in a transcription-independent manner.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!