Premise Of The Study: Microsatellite primers were developed in the perennial herbs of the diploid-polyploid complex Veronica subsect. Pentasepalae (Plantaginaceae) to investigate the role that hybridization has played in the evolution of the group, which includes several endangered species.
Methods And Results: Twelve pairs of primers leading to polymorphic and readable markers were identified and optimized from V. jacquinii and V. orbiculata using a microsatellite-enriched library method and 454 GS-FLX technique. The set of primers amplified dinucleotide to pentanucleotide repeats, and the number of alleles per locus ranged from one to six, one to 11, and one to nine for V. orsiniana, V. javalambrensis, and V. rosea, respectively. Transferability analyses were performed in 20 species corresponding to 10 different subgenera.
Conclusions: These results indicate the utility of the newly developed microsatellites across Veronica subsect. Pentasepalae, which will help in the study of gene flow patterns and genetic structure.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4610313 | PMC |
| http://dx.doi.org/10.3732/apps.1500059 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Environmental differences are correlated with the distribution pattern of cytotypes in Veronica subsection Pentasepalae at a broad scale.
Ann Bot
March 2020
Departamento de Botánica, University of Salamanca, Salamanca, Spain.
Background And Aims: The distribution of cytotypes and its potential correlation with environmental variables represent a cornerstone to understanding the origin and maintenance of polyploid lineages. Although many studies have addressed this question in single species at a regional scale, only a few have attempted to decipher this enigma in groups of closely related species at a broad intercontinental geographical scale. Here, we consider approx.
View Article and Find Full Text PDFDivide and conquer! Data-mining tools and sequential multivariate analysis to search for diagnostic morphological characters within a plant polyploid complex (Veronica subsect. Pentasepalae, Plantaginaceae).
PLoS One
December 2018
Departamento de Botánica y Biobanco de ADN vegetal, Universidad de Salamanca, Salamanca, Spain.
This study exhaustively explores leaf features seeking diagnostic characters to aid the classification (assigning cases to groups, i.e. populations to taxa) in a polyploid plant-species complex.
View Article and Find Full Text PDFThe challenge of species delimitation in the diploid-polyploid complex Veronica subsection Pentasepalae.
Mol Phylogenet Evol
February 2018
Departamento de Botánica, University of Salamanca, E-37007 Salamanca, Spain; Biobanco de ADN Vegetal, University of Salamanca, Edificio Multiusos I+D+i, Calle Espejo s/n, 37007 Salamanca, Spain.
A reliable taxonomic framework and the identification of evolutionary lineages are essential for effective decisions in conservation biodiversity programs. However, phylogenetic reconstruction becomes extremely difficult when polyploidy and hybridization are involved. Veronica subsection Pentasepalae is a diploid-polyploid complex of ca.
View Article and Find Full Text PDFCharacterization of 12 polymorphic SSR markers in Veronica subsect. Pentasepalae (Plantaginaceae) and cross-amplification in 10 other subgenera.
Appl Plant Sci
October 2015
Departamento de Botánica, Universidad de Salamanca, E-37007 Salamanca, Spain ; Biobanco de ADN Vegetal, Banco Nacional de ADN, Edificio Multiusos I+D+i, Calle Espejo s/n, 37007 Salamanca, Spain.
Premise Of The Study: Microsatellite primers were developed in the perennial herbs of the diploid-polyploid complex Veronica subsect. Pentasepalae (Plantaginaceae) to investigate the role that hybridization has played in the evolution of the group, which includes several endangered species.
Methods And Results: Twelve pairs of primers leading to polymorphic and readable markers were identified and optimized from V.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!