Premise Of The Study: Rehmannia glutinosa (Scrophulariaceae) is used in traditional Chinese medicine. Microsatellite primers were developed and characterized for this species to evaluate its genetic diversity and population genetic structure.
Methods And Results: Sixteen microsatellite loci were isolated from R. glutinosa using an enriched genomic library, and these markers were characterized in two wild populations of this species. The number of alleles per locus ranged from two to 20. A high genetic diversity was observed in two populations, with average observed heterozygosity of 0.812 and 0.794, and average expected heterozygosity of 0.802 and 0.814, respectively.
Conclusions: Rehmannia glutinosa is an important medicinal resource. The genetic markers described in our study will be useful for future population genetic studies and molecular breeding programs on this species.
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http://dx.doi.org/10.3732/apps.1500054 | DOI Listing |
Zhongguo Zhong Yao Za Zhi
November 2024
School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China.
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September 2024
School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046, China Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials Zhengzhou 450046, China.
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View Article and Find Full Text PDFAnal Chem
December 2024
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, Nanchang 330013, P. R. China.
The pharmacological effects of traditional Chinese medicine (TCM) are closely associated with the processing procedures, which involve a multitude of intricate chemical reactions. Real-time monitoring of the dynamic changes in chemical constituents during TCM processing plays a pivotal role in ensuring quality control of TCM products. Herein, a neutral desorption atmospheric pressure chemical ionization mass spectrometry (ND-APCI-MS) method was developed for online monitoring of the TCM processing.
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December 2024
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address:
Rehmanniae Radix Preparata, a processed form of the traditional Chinese medicinal plant Rehmannia glutinosa Libosch, has long been valued for its medicinal properties and use as a food. It is notably effective in treating postmenopausal osteoporosis. This study utilized C18 to separate and purify different concentrations of its eluent streams.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.
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