AI Article Synopsis
- A new method for quantitatively measuring folates in rodent diets was developed to ensure accurate interpretation of folate intake during feeding trials, addressing inconsistencies in diet folate levels compared to product labels.
- The method utilizes liquid chromatography tandem-mass spectrometry to differentiate and quantify five specific folate species with a detection range from 8 μg to 2 mg/kg feed, following FDA guidelines for validation.
- The effectiveness of this method was demonstrated through the analysis of various feed samples, examining the impact of casein, and assessing variability in folate content across different production batches.
Article Abstract
A method for the quantitative determination of folates in rodent diets is very important for correct interpretation of folate intake during feeding trials, given the possible discrepancy between the actual folate concentration in the diet and that mentioned on the product sheet. Liquid chromatography tandem-mass spectrometry is the method of choice to differentiate and quantify the individual folate species present. This discrepancy may be accounted for by, e.g., inaccurate folic acid supplementation and/or the presence of endogenous reduced and substituted folates. We developed a method, validated based on FDA guidelines, that allows the measurement of added and endogenous folates by quantitative determination of 5 folate monoglutamates with linear ranges from 8 μg to 2 mg/kg feed. This information, combined with feed intake data, allows insight into the actual folate intake in animal feeding studies. The relevance of this method was illustrated by the analysis of several feed samples of varying composition, by the investigation of the effect of casein incorporation, and by evaluating the variability of the folate content between pellets and production batches.
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| http://dx.doi.org/10.1021/acs.jafc.5b04075 | DOI Listing |
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