A ratiometric two-photon fluorescent probe was developed based on quinoline platform to detect hydrazine (NH) with high selectivity. exhibited large two-photon absorption cross sections at 710 nm (250 GM) and excellent ratiometric two-photon fluorescent detection signal for hydrazine. was also successfully applied to selectively detect hydrazine vapor even at a concentration down to 0.05%. Cell cytotoxicity and bio-imaging studies revealed that probe was cell-permeable and could be used to detect hydrazine in living cells with low cytotoxicity under two-photon excitation.
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http://dx.doi.org/10.1016/j.snb.2015.07.051 | DOI Listing |
Chem Commun (Camb)
January 2025
College of Biological, Chemical Science and Engineering, Jiaxing University, Jiaxing 314001, China.
We developed a novel ratiometric two-photon fluorescent probe for the selective and precise detection of nitroreductase (NTR) in neurons. Using this probe, we found that hypoxic stimulation could up-regulate endogenous NTR levels.
View Article and Find Full Text PDFAdv Healthc Mater
November 2024
Anhui Provincial Key Laboratory of Biomedical Materials and Chemical Measurement, Laboratory of Functionalized Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241002, China.
Alzheimer's disease (AD) is characterized by multiple toxicity from various biomarkers, and it is very important to monitor the fluctuation of biomarker level in brain tissues to track its early onset, illness progression, and therapeutic effect. Hypochlorous acid (HOCl) is confirmed to be a reliable biomarker to extend the scope of diagnosis. However, the practical applications of the developed conjugated small molecule fluorescent probes for detecting HOCl are often restricted to a large extent by their low solubility, aggregation-caused quenching (ACQ), unsatisfactory fluorescence brightness, small Strokes-shift, and lack of self-correcting ratiometric emitters.
View Article and Find Full Text PDFAnal Chem
October 2024
Zhejiang Engineering Research Center of Advanced Mass Spectrometry and Clinical Application, Institute of Mass Spectrometry, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China.
Anal Chem
October 2024
Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China.
A luminescent nanoprobe capable of orthogonal sensing of two independent events is highly significant for unbiased disease-related detection such as the detection of senescent cells. Moreover, it is invaluable that the nanoprobe possesses a two-photon excitable characteristic that is highly suitable for imaging living cells and tissues. Herein, we present a two-photon-excitable multiluminescent orthogonal-sensing nanoprobe (OS nanoprobe) capable of detecting both pH elevation and β-galactosidase (β-gal) overexpression in senescent cells.
View Article and Find Full Text PDFACS Appl Mater Interfaces
September 2024
School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.
The dysregulation of pH has been linked to the onset of chronic conditions, such as cancer and neurological diseases. Consequently, the development of a highly sensitive tool for intracellular pH sensing is imperative to investigate the interplay between pH and the biochemical changes accompanying disease pathogenesis. Here, we present the development of a ratiometric fluorescent nanoprobe, , designed for precisely measuring pH levels.
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