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Evaluation of Stress Tolerance and Fermentation Performance in Commercial Yeast Strains for Industrial Applications.
Foods
January 2025
Science Center for Future Foods, Jiangnan University, Wuxi 214122, China.
This study evaluates the stress tolerance and metabolic adaptability of twelve yeast strains, including eleven commercial strains from Wyeast Laboratories and one prototrophic laboratory strain, under industrially relevant conditions. Yeast strains were assessed for their fermentation performance and stress responses under glucose limitation, osmotic stress, acid stress, elevated ethanol concentrations, and temperature fluctuations. Results revealed significant variability in glucose consumption, ethanol production, and stress tolerance across strains.
View Article and Find Full Text PDFEffects of Gene on Desiccation Resistance in .
Microorganisms
November 2024
State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
, an opportunistic foodborne pathogen, has a strong resistance to osmotic stress and desiccation stress, but the current studies cannot elucidate this resistance mechanism absolutely. A mechanosensitive channel MscM was suspected of involving to desiccation resistance mechanism of To investigate the specific molecular mechanism, the mutant strain (Δ) was constructed using the homologous recombination method, and the complementary strain was obtained by gene complementation, followed by the analysis of the difference between the wild-type (WT), mutant, and complementary strains. Compared to the wild-type bacteria (WT), the inactivation rate of the Δ strain decreased by 15.
View Article and Find Full Text PDFInactivation of Lipopolysaccharide-Biosynthesizing Genes Altered Lipids Composition and Intensity in .
Pathogens
November 2024
Guangzhou CnFerment Biotechnology Co., Ltd., Guangzhou 510440, China.
Gram-negative bacteria possess an asymmetric outer membrane, where the outer leaflet consists of LPSs and the inner leaflet comprises phospholipids. , an opportunistic milk-borne pathogen that causes severe neonatal meningitis and bacteremia, displays diverse lipopolysaccharide (LPS) structures. As a barrier of the bacterial cell, LPSs likely influenced resistance to environment stresses; however, there are no research reports on this aspect, hindering the development of novel bactericidal strategies overcoming the pathogen's resilience.
View Article and Find Full Text PDFEntropy generation and water conservation in the mammalian nephron.
J Comp Physiol B
January 2025
Departamento de Fisiologia, Instituto de Biociências da Universidade de São Paulo, São Paulo, Brazil.
During the transition from fresh waters to terrestrial habitats, significant adaptive changes occurred in kidney function of vertebrates to cope with varying osmotic challenges. We investigated the mechanisms driving water conservation in the mammalian nephron, focusing on the relative contributions of active ion transport and Starling forces. We constructed a thermodynamic model to estimate the entropy generation associated with different processes within the nephron, and analyzed their relative importance in urine formation.
View Article and Find Full Text PDFSynergistic effect of alkane and membrane lipid alteration in Synechococcus elongatus PCC 7942 under salt and light stresses.
J Plant Res
December 2024
Graduate School of Science and Technology, Shizuoka University, Suruga-ku, Shizuoka, 422-8529, Japan.
Salinity and light markedly influence cyanobacterial viability. High salinity disrupts the osmotic balance, while excess light energy affects redox potential in the cells. Regulating the ratio of saturated and unsaturated alka(e)ne and fatty acids in cyanobacteria is thought to have crucial roles in coping with these stresses by regulating membrane fluidity.
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