Objective: To investigate the chemical constituents from the fruits of Paulownia tomentosa.

Methods: The compounds were isolated and purified by column chromatography and their structures were identified on the basis of physicochemical properties and spectral analyses.

Results: Seven compounds were isolated and their structures were identified as ursolic acid (1), sesamin(2),2α,3α, 19a-trihydroxyurs-12-en-28-oic acid(3), luteolin(4), tricin-7-O-β-D-glucoside(5),3',4',5,7-tetrahydroxy-6-[7-hydroxy-3,7-dimethyl-2(E)-octenyl] flavanone(6) and stigmasterol(7).

Conclusion: Compounds 3, 5 and 7 are isolated from Paulownia genus for the first time. Compound 2 is isolated from Paulownia tomentosa for the first time.

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