AI Article Synopsis
- The study investigates how G-tetraplex structures in human telomeric sequences can induce fluorescence resonance energy transfer (FRET) with a specially designed nucleoside pair.
- By transitioning from a single strand to an anti-parallel G-quadruplex, energy is transferred from a donor molecule (pyrene) to an acceptor molecule (perylene), demonstrating the potential for this mechanism in biological applications.
- The research shows that increasing the number of G-quartet spacers decreases FRET efficiency, suggesting implications for developing advanced FRET-based sensing methods.
Article Abstract
G-tetraplex induced fluorescence resonance energy transfer (FRET) within telomeric repeat sequences has been studied using a nucleoside-tethered FRET pair embedded in the human telomeric G-quadruplex forming sequence (5'-A GGG TT(Py) A GGG TT(Per) A GGG TTA GGG-3', Py=pyrene, Per=perylene). Conformational change from a single strand to an anti-parallel G-quadruplex leads to FRET from energy donor ((Py) A) to acceptor ((Per) A). The distance between the FRET donor/acceptor partners was controlled by changing the number of G-quartet spacer units. The FRET efficiency decreases with increase in G-quartet units. Overall findings indicate that this could be further used for the development of FRET-based sensing and measurement techniques.
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| http://dx.doi.org/10.1002/asia.201500996 | DOI Listing |
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