AI Article Synopsis
- DNA double-strand breaks (DSBs) play a crucial role in various biological processes across different organisms, influencing both normal functions and disease states.
- A new method has been developed for mapping these breaks at a detailed level, leading to the identification of large DNA fragments known as 'forum domains'.
- The study focuses on the mapping of DSBs within repetitive rDNA units in human HEK293T cells using Illumina sequencing, providing comprehensive experimental details and bioinformatics analyses that have been shared publicly in the Gene Expression Omnibus.
Article Abstract
DNA double-strand breaks (DSBs) are associated with different physiological and pathological processes in different organisms. To understand the role of DSBs in multiple cellular mechanisms, a robust method for genome-wide mapping of chromosomal breaks at one-nucleotide resolution is required. Many years ago, we detected large DNA fragments migrating from DNA-agarose plugs in pulsed-field gels, which we named 'forum domains' [1,2]. Recently, we developed a method for genome-wide mapping of DSBs that produces these 50-150 kb DNA domains using microarrays or 454 sequencing (Tchurikov et al., 2011; 2013). Now we have used Illumina sequencing to map DSBs in repetitive rDNA units in human HEK293T cells. Here we describe in detail the experimental design and bioinformatics analysis of the data deposited in the Gene Expression Omnibus with accession number GSE49302 and associated with the study published in the Journal of Molecular Cell Biology (Tchurikov et al., 2014).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4535614 | PMC |
| http://dx.doi.org/10.1016/j.gdata.2014.10.024 | DOI Listing |
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