Endosperm is a product of double fertilization, and provides nutrients and signals to the embryo during seed development in flowering plants. Early stages of endosperm development are critical for the development of its storage capacity through synthesis and accumulation of starch and storage proteins. Here we report on the isolation and sequencing of mRNAs from the central portion of the starchy endosperm of Zea mays (maize) B73 at 6 days after pollination. We detected a high level of correlation among the four biological replicates of RNAs isolated using laser-capture microdissection of the cell type. Because the assayed developmental stage precedes the synthesis and accumulation of the major storage proteins and starch in the endosperm, our dataset likely include mRNAs for genes that are involved in control and establishment of these storage programs. The mRNA-Seq data has been deposited in Gene Expression Omnibus (accession number GSE58504).
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http://dx.doi.org/10.1016/j.gdata.2014.07.003 | DOI Listing |
Plant Physiol Biochem
December 2024
Biobank, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China; Department of Clinical Medicine, Southwest Medical University, Luzhou, Sichuan, China.
A diet rich in anthocyanins can benefit human health against a broad spectrum of human diseases due to the high antioxidant activities of anthocyanins. Enrichment of anthocyanins in the starchy endosperm of rice is an effective solution to provide nutritional food in human diets. However, previous attempts failed to engineer anthocyanin biosynthesis in the rice endosperm by transgenic expression of rice endogenous genes.
View Article and Find Full Text PDFInt J Phytoremediation
November 2024
Department of Botany, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, India.
Food Funct
December 2024
U.S. Department of Agriculture, Western Regional Research Center, Agricultural Research Service, Albany, CA, USA.
Black rice bran, a waste product from the commercial milling of black rice that removes the bran and germ leaving the starchy endosperm, contains bioactive anthocyanin, phenolic, and phytosteroid compounds that may have health benefits. This study determined the effect of a polysaccharide-rich bioprocessed (fermented) black rice bran and a green tea extract individually and in combination on weight loss in orally fed mice on a high-fat diet and on concurrent changes in blood glucose and insulin as well as in cholesterol, triglyceride, and high-density and low-density lipoproteins (HDL and LDL). At the end of the eight-week feeding study, the combination diet resulted in a 67% lower weight gain than mice on a high-fat diet alone, a greater effect than that of bioprocessed black rice bran or green tea extract individually.
View Article and Find Full Text PDFMethods Mol Biol
November 2024
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.
RNA extraction is a critical step in gene expression analysis. While numerous RNA extraction kits are commercially available, most kits cannot be utilized for RNA extraction from rice endosperm that contains abundant starch. Here, I describe a three-step RNA extraction from rice starchy endosperm.
View Article and Find Full Text PDFInt J Biol Macromol
November 2024
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014, China. Electronic address:
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