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Disulfide-Bridged Cleavable PEGylation of Poly-L-Lysine for SiRNA Delivery. | LitMetric

Disulfide-Bridged Cleavable PEGylation of Poly-L-Lysine for SiRNA Delivery.

Methods Mol Biol

Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China.

Published: April 2016

Engineered PEG-cleavable catiomers based on poly-L-lysine have been developed as nonviral gene vectors, which have been found to be one of important methods to balance "PEG dilemma." In this protocol, we aim at the standardization of the method and procedure of PEG-cleavable catiomers. Major steps including ring-opening polymerization (ROP) of ε-benzyloxycarbonyl-L-lysine N-carboxyanhydride (zLL-NCA) monomers to yield PEG-cleavable polylysine, examination on bio-stability and bio-efficacy of its gene complexes are described.

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Source
http://dx.doi.org/10.1007/978-1-4939-3112-5_5DOI Listing

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