AI Article Synopsis
- The study aimed to explore how AHNAK influences fat cell development (adipogenesis) and glucose regulation, especially in the context of obesity.
- Researchers conducted experiments on stem cells and used AHNAK-deficient mice on a high-fat diet to observe effects on metabolism and body fat.
- Results showed that a lack of AHNAK disrupted fat cell development but improved metabolic health in mice, indicating its important role in managing body fat and metabolism.
Article Abstract
Objective: Recent evidence has suggested that AHNAK expression is altered in obesity, although its role in adipose tissue development remains unclear. The objective of this study was to determine the molecular mechanism by which Ahnak influences adipogenesis and glucose homeostasis.
Design: We investigated the in vitro role of AHNAK in adipogenesis using adipose-derived mesenchymal stem cells (ADSCs) and C3H10T1/2 cells. AHNAK-KO male mice were fed a high-fat diet (HFD; 60% calories from fat) and examined for glucose and insulin tolerances, for body fat compositions, and by hyperinsulinemic-euglycemic clamping. Energy expenditures were assessed using metabolic cages and by measuring the expression levels of genes involved in thermogenesis in white or brown adipose tissues.
Results: Adipogenesis in ADSCs was impaired in AHNAK-KO mice. The loss of AHNAK led to decreased BMP4/SMAD1 signaling, resulting in the downregulation of key regulators of adipocyte differentiation (P<0.05). AHNAK directly interacted with SMAD1 on the Pparγ2 promoter. Concomitantly, HFD-fed AHNAK-KO mice displayed reduced hepatosteatosis and improved metabolic profiles, including improved glucose tolerance (P<0.001), enhanced insulin sensitivity (P<0.001), and increased energy expenditure (P<0.05), without undergoing alterations in food intake and physical activity.
Conclusion: AHNAK plays a crucial role in body fat accumulation by regulating adipose tissue development via interaction with the SMAD1 protein and can be involved in metabolic homeostasis.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605776 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139720 | PLOS |
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