AI Article Synopsis

  • - Nodal is found to be highly expressed in various human cancers, making it a promising target for therapy, and a new monoclonal antibody (mAb), 3D1, has been developed to target Nodal specifically.
  • - In laboratory tests, treatment with 3D1 mAb reduced tumor growth and key signaling proteins in melanoma cells, indicating its effectiveness in inhibiting cancer progression.
  • - The study also demonstrated that 3D1 mAb decreased tumor growth in mouse models and developed a method to detect Nodal levels in cancer patients' serum, suggesting its potential for targeted cancer therapy.

Article Abstract

Nodal is highly expressed in various human malignancies, thus supporting the rationale for exploring Nodal as a therapeutic target. Here, we describe the effects of a novel monoclonal antibody (mAb), 3D1, raised against human Nodal. In vitro treatment of C8161 human melanoma cells with 3D1 mAb shows reductions in anchorage-independent growth and vasculogenic network formation. 3D1 treated cells also show decreases of Nodal and downstream signaling molecules, P-Smad2 and P-ERK and of P-H3 and CyclinB1, with an increase in p27. Similar effects were previously reported in human breast cancer cells where Nodal expression was generally down-regulated; following 3D1 mAb treatment, both Nodal and P-H3 levels are reduced. Noteworthy is the reduced growth of human melanoma xenografts in Nude mice treated with 3D1 mAb, where immunostaining of representative tumor sections show diminished P-Smad2 expression. Similar effects both in vitro and in vivo were observed in 3D1 treated A375SM melanoma cells harboring the active BRAF(V600E) mutation compared to treatments with IgG control or a BRAF inhibitor, dabrafenib. Finally, we describe a 3D1-based ELISA for the detection of Nodal in serum samples from cancer patients. These data suggest the potential of 3D1 mAb for selecting and targeting Nodal expressing cancers.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741437PMC
http://dx.doi.org/10.18632/oncotarget.6049DOI Listing

Publication Analysis

Top Keywords

3d1 mab
16
effects novel
8
monoclonal antibody
8
nodal
8
human melanoma
8
melanoma cells
8
3d1 treated
8
3d1
7
human
5
mab
5

Similar Publications

Drug development in recent years is increasingly focused on developing personalized treatments based on blocking molecules selective for therapeutic targets specifically present in individual patients. In this perspective, the specificity of therapeutic targets and blocking agents plays a crucial role. Monoclonal antibodies (mAbs) and their surrogates are increasingly used in this context thanks to their ability to bind therapeutic targets and to inhibit their activity or to transport bioactive molecules into the compartments in which the targets are expressed.

View Article and Find Full Text PDF

Gold nanoparticle-based immunoassay for the detection of bifenthrin in vegetables.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess

March 2022

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

We have developed a sensitive and rapid gold nanoparticle-based immunochromatographic strip (GNP-ICS) for the detection of bifenthrin (BF) using an anti-BF monoclonal antibody (mAb). When used in indirect competitive enzyme-linked immunosorbent assay (ELISA), the specific anti-BF mAb (3D1) had a half-maximal inhibitory concentration (IC) and limit of detection (LOD) of 59 and 15 ng mL respectively. Additionally, its cross-reactivity (CR) with other pyrethroids was negative.

View Article and Find Full Text PDF

Insights into nitrogen removal from seawater-based wastewater through marine anammox bacteria under ampicillin stress: Microbial community evolution and genetic response.

J Hazard Mater

February 2022

National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Global spread of ampicillin (AMP) in the aquatic environment have attracted much attention recently. Marine anammox bacteria (MAB) have potentials in saline wastewater treatment due to their good salt tolerance. However, to date, the effect resulting from AMP on MAB is still unknown.

View Article and Find Full Text PDF

Development of an efficient neutralization assay for Coxsackievirus A10.

Appl Microbiol Biotechnol

February 2019

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, 361102, People's Republic of China.

Coxsackievirus A10 (CVA10) recently has become one of the major pathogens of hand, foot, and mouth disease (HFMD) in children worldwide, but no cure or vaccine against CVA10 is available yet. Serological evaluation of herd immunity to CVA10 will promote the development of vaccine. The traditional neutralization assay based on inhibition of cytopathic effect (Nt-CPE) is a common method for measuring neutralizing antibody titer against CVA10, which is time-consuming and labor-intensive.

View Article and Find Full Text PDF

Mucin 1 (MUC1) is a heterodimeric protein that is aberrantly overexpressed on the surface of diverse human carcinomas and is an attractive target for the development of mAb-based therapeutics. However, attempts at targeting the shed MUC1 N-terminal subunit have been unsuccessful. We report here the generation of mAb 3D1 against the nonshed oncogenic MUC1 C-terminal (MUC1-C) subunit.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: