A highly selective phosphorescence probe for histidine in living bodies.

Dalton Trans

State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116024, China.

Published: November 2015

In this work, we designed and synthesized a heterobimetallic ruthenium(ii)-nickel(ii) complex, [Ru(bpy)2(phen-DPA)Ni](PF6)4 (Ru-Ni), as a highly selective phosphorescence probe for histidine. The probe exhibited weak emission at 603 nm because the phosphorescence of the Ru(ii) complex can be strongly quenched by the paramagnetic Ni(2+) ion. In the presence of histidine, reaction of Ru-Ni with histidine resulted in the release of nickel(ii) and an enhancement in the phosphorescence intensity at 603 nm. Ru-Ni showed high selectivity for histidine even in the presence of other amino acids and cellular abundant species. Cell imaging experimental results demonstrated that Ru-Ni is membrane permeable, and can be applied for visualizing histidine in live cells. More interestingly, Ru-Ni also can act as a novel reaction-based nuclear staining agent for visualizing exclusively the nuclei of living cells with a significant phosphorescence enhancement. In addition, the potential of the probe for biological applications was confirmed by employing it for phosphorescence imaging of histidine in larval zebrafish and Daphnia magna. These results demonstrated that Ru-Ni would be a useful tool for physiological and pathological studies involving histidine.

Download full-text PDF

Source
http://dx.doi.org/10.1039/c5dt03319eDOI Listing

Publication Analysis

Top Keywords

highly selective
8
selective phosphorescence
8
phosphorescence probe
8
histidine
8
probe histidine
8
demonstrated ru-ni
8
phosphorescence
6
ru-ni
6
probe
4
histidine living
4

Similar Publications

Behavioral Profiling in Zebrafish Identifies Insecticide-Related Compounds.

J Agric Food Chem

January 2025

Institute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, California 94158, United States.

Pesticides, including insecticides, are indispensable for large-scale agriculture. Modulating chloride ion channels has proven highly successful as a mode of action (MoA) for insect management. Identifying new ligands for these channels affords opportunities for the potential development of new insecticide products.

View Article and Find Full Text PDF

Palladium (Pd) catalysts are promising for electrochemical reduction of CO to CO but often can be deactivated by poisoning owing to the strong affinity of *CO on Pd sites. Theoretical investigations reveal that different configurations of *CO endow specific adsorption energies, thereby dictating the final performances. Here, a regulatory strategy toward *CO absorption configurations is proposed to alleviate CO poisoning by simultaneously incorporating Cu and Zn atoms into ultrathin Pd nanosheets (NSs).

View Article and Find Full Text PDF

Understanding the naïve B cell repertoire and its specificity for potential zoonotic threats, such as the highly pathogenic avian influenza (HPAI) H5Nx viruses, may allow prediction of infection- or vaccine-specific responses. However, this naïve repertoire and the possibility to respond to emerging, prepandemic viruses are largely undetermined. Here, we profiled naïve B cell reactivity against a prototypical HPAI H5 hemagglutinin (HA), the major target of antibody responses.

View Article and Find Full Text PDF

Objective: To evaluate and compare the readability of information on different treatment options for breast cancer from WeChat public accounts, propose targeted improvement strategies based on the evaluation of the results of the various treatment options, and provide a reference for producers of WeChat public accounts from which to write highly readable information regarding breast cancer treatment options.

Methods: With "breast cancer" as keywords in April 2021, searches were implemented on Sogou WeChat website (https://weixin.sogou.

View Article and Find Full Text PDF

The study analyzed the spatial distribution characteristics, evolution rules, and driving factors of 138 China's national agricultural cultural heritage sites from 2013 to 2021 at the overall and regional levels, using kernel density analysis, Centres for standard deviation ellipse analyses, spatial autocorrelation analysis, and geographical detector analysis.The results showed that: ①From an overall perspective, the spatial pattern of China's national agricultural cultural heritage changed greatly from 2013 to 2021, with a highly uneven spatial distribution, gradually showing a distribution pattern of "widely distributed, locally concentrated". The spatial distribution of China's national agricultural cultural heritage is increasingly evident, and the spatial distribution type has evolved from discrete to clustered.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!