AI Article Synopsis
- High-throughput live-cell screens are crucial for systems biology and drug discovery, allowing researchers to study cell behavior and interactions effectively.
- The study presents a new method using optogenetics, which eliminates the need for chemical agents, streamlines procedures, and enhances data quality in small-molecule screening for human protein kinases.
- This all-optical screening approach is versatile and can be tailored for various drug targets and cellular mechanisms.
Article Abstract
High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652335 | PMC |
| http://dx.doi.org/10.1038/nchembio.1933 | DOI Listing |
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