Over 50 million hogs are raised annually in the United States for consumption, mostly on industrial hog operations (IHOs). Workers at IHOs are exposed to airborne particulates, zoonotic pathogens, and other workplace hazards, but lack of access to IHOs can hinder exposure assessment in epidemiologic studies. Here, we demonstrate the utility of pig-specific Bacteroidales (Pig-2-Bac) as a biomarker of exposure to pigs and pig waste and to help identify sources of Staphylococcus aureus carriage among IHO workers.
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http://dx.doi.org/10.1016/j.envres.2015.09.026 | DOI Listing |
Sci Rep
August 2024
Department of Environmental, Water and Earth Sciences, Arcadia Campus, Tshwane University of Technology, 175 Nelson Mandela Avenue, Arcadia, Pretoria, 0001, South Africa.
Access to safe drinking water sources and appropriate sanitation facilities remains a dream in low and middle-income countries including South Africa. This study identified the origin of faecal pollution by using quantitative polymerase chain reaction (qPCR) targeting host-specific Bacteroidales genetic markers to track the distribution of human-specific (BacHum) and animal-specific (cattle-BacCow, chicken-Cytb, pig-Pig-2-Bac, dog-BacCan) markers in water sources used by rural communities of the Vhembe District Municipality (VDM). Results revealed the prevalence of BacHum, BacCow, and BacCan in all surface water sources in Thulamela Local Municipality (TLM) and Collins Chabane Local Municipality (CLM) during wet (100%) and dry seasons (50-75%).
View Article and Find Full Text PDFSci Total Environ
January 2024
Tshwane University of Technology, Department of Environmental, Water and Earth Sciences, Arcadia Campus, P/B X 680, Pretoria 0001, South Africa. Electronic address:
Sci Total Environ
November 2020
Division of Infectious Diseases, University of Virginia, Charlottesville, VA, USA; Asociacion Benefica Prisma, Iquitos, Peru. Electronic address:
The performance of eight microbial source tracking (MST) markers was evaluated in a low-resource, tropical community located in Iquitos, Peru. Fecal samples from humans, dogs, cats, rats, goats, buffalos, guinea-pigs, chickens, ducks, pigeons, and parrots were collected (n = 117). All samples were tested with human (BacHum, HF183-Taqman), dog (BactCan), pig (Pig-2-Bac), and avian (LA35, Av4143, ND5, cytB) markers using quantitative PCR (qPCR).
View Article and Find Full Text PDFEnviron Pollut
May 2018
Research Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok, Thailand; Center of Excellence on Environmental Health and Toxicology (EHT), Ministry of Education, Bangkok, Thailand. Electronic address:
Microbial source tracking (MST) DNA-based assays have been used to successfully solve fecal pollution problems in many countries, particularly in developed nations. However, their application in developing countries has been limited but continues to increase. In this study, sixteen endpoint and quantitative PCR (qPCR) assays targeting universal and human-, swine-, and cattle-specific Bacteroidales gene markers were modified for endpoint PCR, evaluated for their performance with sewage and fecal samples from the Tha Chin watershed and subsequently validated with samples from the Chao Phraya watershed, Thailand.
View Article and Find Full Text PDFSci Rep
October 2016
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, China.
This study systematically evaluated five microbial and four mitochondrial DNA (mtDNA) markers, including sensitivities and specificities under PCR method, and fecal concentrations and decay rates in water under qPCR method. The microbial DNA markers were the three human-associated (BacH, HF183 and B.adolescentis) and two pig-associated (Pig-2-Bac and L.
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