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Quantitative analysis of Δ- and Δ-tetrahydrocannabinol metabolites and isomers: a rapid assay in urine by LC-MS/MS.
Anal Bioanal Chem
January 2025
Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA.
An increasing number of cannabis-related products have become available and entered the market, particularly those containing cannabidiol (CBD) and Δ-tetrahydrocannabinol (Δ-THC). Analytical methods for cannabinoids in urine have been described extensively in the literature. However, methods providing good resolution for distinguishing interferences from THC positional isomers are needed.
View Article and Find Full Text PDFLipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum.
Front Mol Biosci
January 2025
Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Introduction: Chronic inflammation caused by infections has a significant negative impact on the reproductive system and impairs fertility. The corpus luteum (CL) plays a central role not only in regulating the ovary cycle, but also in implantation of the embryo and maintenance of early pregnancy through the secretion of progesterone. Understanding the intricate interplay between inflammatory processes and reproductive organ's function is crucial for the development of effective therapeutic strategies to alleviate reproductive disorders and improve fertility.
View Article and Find Full Text PDFFitness costs of Mycobacterium tuberculosis resistant to rifampicin is compensated by rapid Th2 polarization mediated by early and high IL-4 production during mice infection.
Sci Rep
January 2025
Experimental Pathology Department, National Institute of Medical Sciences and Nutrition Salvador Zubiran, Mexico City, Mexico.
It was a general belief that drug resistance in Mycobacterium tuberculosis (Mtb) was associated with lesser virulence, particularly rifampicin resistance, which is usually produced by mutations in the RNA polymerase Beta subunit (RpoB). Interestingly, this kind of bacterial mutations affect gene transcription with significant effects on bacterial physiology and metabolism, affecting also the bacterial antigenic constitution that in consequence can produce diverse immune responses and disease outcome. In the present study, we show the results of the Mtb clinical isolate A96, which is resistant to rifampicin and when used to infect BALB/c mice showed hypervirulence, apparently by rapidly polarization of the Th2 immune response through early and high production of IL-4.
View Article and Find Full Text PDFFilter-aided extracellular vesicle enrichment (FAEVEr) for proteomics.
Mol Cell Proteomics
January 2025
VIB-UGent Center for Medical Biotechnology, VIB, 9052 Ghent, Belgium; Department of Biomolecular Medicine, Ghent University, 9052 Ghent, Belgium. Electronic address:
Extracellular vesicles (EVs), membrane-delimited nanovesicles that are secreted by cells into the extracellular environment, are gaining substantial interest due to their involvement in cellular homeostasis and their contribution to disease pathology. The latter in particular has led to an exponential increase in interest in EVs as they are considered to be circulating packages containing potential biomarkers and are also a possible biological means to deliver drugs in a cell-specific manner. However, several challenges hamper straightforward proteome analysis of EVs as they are generally low abundant and reside in complex biological matrices.
View Article and Find Full Text PDFImpacts of hnRNP A1 Splicing Inhibition on the Brain Remyelination Proteome.
J Neurochem
January 2025
Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, Brazil.
Oligodendrocytes, the myelinating cells in the central nervous system, are implicated in several neurological disorders marked by dysfunctional RNA-binding proteins (RBPs). The present study aimed at investigating the role of hnRNP A1 in the proteome of the corpus callosum, prefrontal cortex, and hippocampus of a murine cuprizone-induced demyelination model. Right after the cuprizone insult, we administered an hnRNP A1 splicing activity inhibitor and analyzed its impact on brain remyelination by nanoESI-LC-MS/MS label-free proteomic analysis to assess the biological processes affected in these brain regions.
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