AI Article Synopsis
- The study investigates how IL-1β influences the expression of ADAMTS-4 and ADAMTS-5 proteins in human nucleus pulposus cells through the NF-κB signaling pathway.
- The researchers used qRT-PCR and Western blot techniques to measure protein levels and conducted gene silencing to explore NF-κB's role in ADAMTS expression and aggrecan degradation.
- The findings indicate that IL-1β boosts ADAMTS expression, and disrupting NF-κB signaling diminishes this effect, highlighting the crucial role of both ADAMTS-4 and ADAMTS-5 in degrading aggrecan in these cells.
Article Abstract
Background: The purpose of this study is to investigate IL-1β regulation of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS-4 and ADAMTS-5) expression through nuclear factor kappa B (NF-κB) in human nucleus pulposus (NP) cells.
Methods: qRT-PCR and Western blot were used to measure ADAMTS expression. Transfections and gene silencing were used to determine the role of NF-κB on cytokine-mediated ADAMTS expression and its role in aggrecan degradation.
Results: IL-1β increased ADAMTS expression in NP cells. Treatment with NF-κB inhibitors abolished the inductive effect of the cytokines on ADAMTS expression. Silencing of p65 confirmed their role in IL-1β-dependent ADAMTS-4 and ADAMTS-5 expression and aggrecan degradation.
Conclusions: By controlling the activation of NF-κB signaling, IL-1β modulates the expression of ADAMTS in NP cells. To our knowledge, this is the first study that shows the contribution of both ADAMTS-4 and ADAMTS-5 to aggrecan degradation in human NP cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594913 | PMC |
| http://dx.doi.org/10.1186/s13018-015-0296-3 | DOI Listing |
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