Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666809 | PMC |
| http://dx.doi.org/10.1038/nmeth.3616 | DOI Listing |
Publication Analysis
Top Keywords
noncontact three-dimensional
4
three-dimensional mapping
4
mapping intracellular
4
intracellular hydromechanical
4
hydromechanical properties
4
properties brillouin
4
brillouin microscopy
4
microscopy current
4
current measurements
4
measurements biomechanical
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!