AI Article Synopsis
- Development of safe and effective delivery vectors is crucial for RNAi-based biotechnologies, and this study presents new dendritic peptide bolaamphiphile vectors that effectively deliver siRNA with low toxicity.
- The research highlights the correlation between molecular design, self-assembled nanostructures, and biological activity, demonstrating that the bolaamphiphile architecture enhances complex stability and transfection efficiency.
- The optimal vector, featuring a fluorocarbon core, showed superior delivery efficiency and serum resistance compared to traditional methods, providing insights for creating better nucleic acid delivery materials and other biomaterial applications.
Article Abstract
Development of safe and effective delivery vectors is a critical challenge for the application of RNA interference (RNAi)-based biotechnologies. In this study we show the rational design of a series of novel dendritic peptide bolaamphiphile vectors that demonstrate high efficiency for the delivery of small interfering RNA (siRNA) while exhibiting low cytotoxicity and hemolytic activity. Systematic investigation into structure-property relationships revealed an important correlation between molecular design, self-assembled nanostructure, and biological activity. The unique bolaamphiphile architecture proved a key factor for improved complex stability and transfection efficiency. The optimal vector contains a fluorocarbon core and exhibited enhanced delivery efficiency to a variety of cell lines and improved serum resistance when compared to hydrocarbon analogues and lipofectamine RNAiMAX. In addition to introducing a promising new vector system for siRNA delivery, the structure-property relationships and "fluorocarbon effect" revealed herein offer critical insight for further development of novel materials for nucleic acid delivery and other biomaterial applications.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582325 | PMC |
| http://dx.doi.org/10.1021/acscentsci.5b00233 | DOI Listing |
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