AI Article Synopsis
- Molecular-based techniques like the FilmArray BCID panel can speed up the diagnosis of infectious diseases, leading to improved patient outcomes.
- The study compared the FilmArray panel results from various clinical specimens with traditional culture methods, finding a 75% agreement and highlighting differences in sensitivity across sample types.
- While the FilmArray BCID panel shows promising results, particularly for abscess samples, further cost-benefit analysis is needed before it can be widely integrated into diagnostic protocols.
Article Abstract
Molecular-based techniques reduce the delay in diagnosing infectious diseases and therefore contribute to better patient outcomes. We assessed the FilmArray blood culture identification (BCID) panel (Biofire Diagnostics/bioMérieux) directly on clinical specimens other than blood: cerebrospinal, joint, pleural and ascitic fluids, bronchoscopy samples and abscesses. We compared the results from 88 samples obtained by culture-based techniques. The percentage of agreement between the two methods was 75 % with a Cohen κ value of 0.51. Global sensitivity and specificity using the FilmArray BCID panel were 71 and 97 %, respectively. Sensitivity was poorer in samples with a low bacterial load, such as ascitic and pleural fluids (25 %), whereas the sensitivity for abscess samples was high (89 %). These findings suggest that the FilmArray BCID panel could be useful to perform microbiological diagnosis directly from samples other than positive blood cultures, as it offers acceptable sensitivity and moderate agreement with conventional microbiological methods. Nevertheless, cost-benefit studies should be performed before introducing this method into algorithms for microbiological diagnostics.
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