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Measuring Cell-Cell Binding Using Flow-Cytometry. | LitMetric

Measuring Cell-Cell Binding Using Flow-Cytometry.

Methods Mol Biol

Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA, 93106, USA.

Published: July 2016

AI Article Synopsis

  • Cell-cell adhesion plays a crucial role in how microbes interact, both competitively and cooperatively.
  • Fluorescence labeling and flow cytometry enable quick and straightforward observation and measurement of these interactions.
  • The described method focuses on quantifying the adhesion events between two populations of cells that are labeled differently.

Article Abstract

Cell-cell adhesion mediates a number of competitive and cooperative microbial interactions. Fluorescence labeling and flow cytometry techniques allow us to observe and measure these interactions rapidly and easily. Here, we describe a method to quantify cell-cell adhesion events between two differentially labeled cell populations.

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Source
http://dx.doi.org/10.1007/978-1-4939-2871-2_9DOI Listing

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