The C. elegans dauer is an attractive model with which to investigate fundamental biological questions, such as how environmental cues are sensed and are translated into developmental decisions through a series of signaling cascades that ultimately result in a transformed animal. Here we describe a simple method of using egg white plates to obtain highly synchronized purified dauers that can be used in downstream applications requiring large quantities of dauers or postdauer animals.
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Article Synopsis
- Nematodes, like Caenorhabditis elegans, experience population cycles and have a survival strategy called diapause, which allows them to produce stress-resistant larvae during tough conditions like starvation.
- The gut microbiome of mixing populations of C. elegans is well-studied, but the microbiome associated with dauer larvae remains largely unexplored, making it interesting for understanding how microbes are passed between nematode generations.
- Research findings show that these dauers typically lack gut bacteria, indicating that the relationship between the host and its microbiome may not be stable over generations, potentially hindering coevolution of the two.
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