Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600740 | PMC |
| http://dx.doi.org/10.1038/ncomms9497 | DOI Listing |
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