Caffeine's influence on object recognition and working-memory in prepubertal mice and its modulation by gender.

Objective: This study investigated the effects of intraperitoneal injection of caffeine on Y-maze working-memory and novel object recognition (NOR) in prepubertal mice.

Methodology: Y-maze spontaneous alternation and a novel object recognition test (consisting of acclimation, acquisition and test phases) were performed. Mice received a single dose of caffeine (10, 20, 40, 80 and 120mgkg(-1) i.p.) or vehicle, 30min before Y-maze exploration. For the NOR test, caffeine was given 30min before training and another dose 30min before test phase.

Results: NOR time (acquisition phase) increased significantly in males at all doses of caffeine and decreased in females at 10, 20 and 40mg/kg compared to vehicle; during the test phase, novel object exploration time decreased significantly in males and increased in females at 10 and 20mg/kg only to decrease again at 120mg/kg. Recognition index decreased in males and increased in females while, males showed poor discrimination between novel and familiar objects compared to vehicle; while females showed increased discrimination between novel and familiar object at 10, 20,40 and 80mg/kg and a decrease at 120mg/kg. Y-maze spontaneous alternation improved significantly in males at 10 and 40mg/kg and decreased at 20 and 120mg/kg in females.

Conclusion: The findings suggest that acute caffeine injection improves non-spatial memory retention in female mice but not in males; spatial working-memory is however improved in males but not in females.