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| http://dx.doi.org/10.1371/journal.pntd.0004089 | DOI Listing |
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A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of and .
Insects
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Corteva Agriscience, 7100 South Dr, Johnston, IA 50131, USA.
and are two species belonging to the Plusiinae subfamily within the Noctuidae family. Due to their morphological similarity, the identification of their larvae is difficult and time-consuming. A rapid and accurate identification of these two species is essential for their management as these species exhibit differential susceptibilities to insecticides and crops engineered to express () proteins, and a molecular tool can easily provide this differentiation.
View Article and Find Full Text PDFVariation of gene ratios in mock communities constructed with purified 16S rRNA during processing.
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Department of Chemical Engineering, Polytechnic School, University of São Paulo, Av. Prof. Luciano Gualberto, Travessa 3, n. 380., São Paulo, SP, CEP 05508-900, Brazil.
16S ribosomal nucleic acid (16S rRNA) analysis allows to specifically target the metabolically active members of microbial communities. The stability of the ratios between target genes in the workflow, which is essential for the bioprocess-relevance of the data derived from this analysis, was investigated using synthetic mock communities constructed by mixing purified 16S rRNA from Bacillus subtilis (Bs), Staphylococcus aureus (Sa), Pseudomonas aeruginosa (Pa), Klebsiella pneumoniae (Kp) and Burkholderia cepacia (Bc) in different proportions. The RT reaction yielded one copy of cDNA per rRNA molecule for Pa, Bc and Sa but only 2/3 of the expected cDNA from 16S rRNAs of Bs and Kp.
View Article and Find Full Text PDFRalstonia pickettii bacteremia: A retrospective review of records.
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Department of Infectious Diseases, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, India. Electronic address:
Introduction: Ralstonia pickettii is a non-fermenting gram-negative bacillus rarely associated with hospital-acquired infections. The study aimed to characterise the clinical profile and outcomes of R. pickettii bacteremia cases.
View Article and Find Full Text PDFIsolation of Macrolactin A from a new Bacillus amyloliquefaciens and its aphicidal activity against Rhopalosiphum padi.
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College of Plant Protection, Northwest A&F University, Yangling, China.
Background: Rhopalosiphum padi is a major infection affecting cereal crops in the boundary area. However, continuous use of chemical pesticides has increased cases of drug resistance in its field population. Therefore, we aimed to verify the insecticidal properties of Bacillus amyloliquefaciens YJNbs21.
View Article and Find Full Text PDFCorrigendum to "Computational analysis to comprehend the structure-function properties of fibrinolytic enzymes from spp for their efficient integration into industrial applications" [Heliyon 10(13), July 2024, e33895].
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Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, 784028, Assam, India.
[This corrects the article DOI: 10.1016/j.heliyon.
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