A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3098
Function: getPubMedXML

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: Attempt to read property "Count" on bool

Filename: helpers/my_audit_helper.php

Line Number: 3100

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3100
Function: _error_handler

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Hirschsprung's Disease in Neonates with Special Reference to Calretinin Immunohistochemistry. | LitMetric

AI Article Synopsis

  • Hirschsprung's disease is a genetic condition caused by a lack of nerve cells in parts of the intestine, diagnosed through clinical, radiological, and histological methods, with Calretinin immunohistochemistry becoming a new diagnostic tool.
  • This study analyzed 89 patients diagnosed with the disease to understand their clinical profiles and evaluate the effectiveness of Calretinin staining in detecting ganglion cells in intestinal biopsies.
  • The results showed Calretinin immunohistochemistry had a 100% sensitivity and 97.44% specificity in diagnosing Hirschsprung's disease, indicating it is a reliable and straightforward method for this condition.

Article Abstract

Background: Hirschsprung's disease is a classic example of a complex genetic disease, characterized by the lack of enteric ganglia in the submucosal and myenteric plexuses, along variable portions of the distal gut. The diagnosis of Hirschsprung's disease is based on a combination of clinical features, radiological appearance of the bowel and histological features in Haematoxylin & Eosin stained sections of intestinal biopsies. Calretinin Immunohistochemistry is emerging to be one of the newer methods.

Aims And Objectives: This study was undertaken to ascertain 1) clinical profile; 2) mode of presentation; 3) to evaluate the role of Calretinin immunostain in the diagnosis of Hirschsprung's Disease.

Materials And Methods: This prospective and observational study was conducted in the Department of Pathology IPGME&R from July 2013 to September 2014. Eighty nine patients, clinically and radiologically diagnosed with Hirschsprung's disease underwent surgery and were included in the study. The data of every patient including age, sex and presenting symptoms were recorded. Eventually, histopathological examination & immunohistochemistry were done.

Results: Total number of cases studied was 89 which aged between 0 days to 28 days. Overall sensitivity in our study to diagnose presence or absence of ganglion cells by calretinin immunohistochemistry was 100% and the specificity is 97.44% with positive and negative predictive value of 84.62 % and 100 % respectively.

Conclusion: Calretinin immunohistochemistry holds several advantages, and it's simple and not doubtful; and it is either positive or negative.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572963PMC
http://dx.doi.org/10.7860/JCDR/2015/12470.6180DOI Listing

Publication Analysis

Top Keywords

hirschsprung's disease
16
calretinin immunohistochemistry
16
diagnosis hirschsprung's
8
positive negative
8
hirschsprung's
5
calretinin
5
immunohistochemistry
5
disease neonates
4
neonates special
4
special reference
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!