Clubroot, caused by Plasmodiophora brassicae, is considered the most devastating soilborne disease in Brassica crops. It has emerged as a serious disease threatening the cruciferous crop production industry in China. Nowadays, the detection techniques for P. brassicae are laborious, time-consuming and low sensitivity. Rapid and effective detection methods are needed. The objective of this study is to develop a Fourier transform infrared spectrometer (FTIR) technique for detection of P. brassicae effectively and accurately. FTIR and Real-time PCR techniques were applied in quantitative detection of P. brassicae. Chinese cabbages were inoculated with P. brassicae. By analyzing the FTIR spectra of P. brassicae, infected clubroots and healthy roots, three specific bands 1 105, 1 145 and 1 228 cm-1 were selected. According to the correlation between the peak areas at these sensitive bands and Real-time PCR Ct value, quantitative evaluation model of P. brassicae was established based on FTIR y=34. 17 +12. 24x - 9. 81x2 - 6. 05x3, r=0. 98 (p<0. 05). To validate accuracy of the model, 10 clubroot samples were selected randomly from field, and detected by FTIR spectrum model, the results showed that the average error is 1. 60%. This demonstrated that the FTIR technology is an available one for the quantitative detection of P. brassicae in clubroot, and it provides a new method for quantitative and quickly detection of Chinese cabbage clubroot.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Bioactive Silk Revolution: Harnessing Curcuminoid Dye and Chitosan for Superior Antimicrobial Defence and UV Shielding.
Pharmaceutics
November 2024
College of Textile Science and Engineering (International Silk Institute), Zhejiang Sci-Tech University, Hangzhou 310018, China.
Background/objectives: The use of natural colourants is gaining attention due to their biocompatibility and functional benefits. This study introduces a different approach using turmeric ( L.) dye extract combined with chitosan to significantly enhance the antibacterial and UV-shielding properties of silk.
View Article and Find Full Text PDFEffect of Process Parameters on Nano-Microparticle Formation During Supercritical Antisolvent Process Using Mixed Solvent: Application for Enhanced Dissolution and Oral Bioavailability of Telmisartan Through Particle-Size Control Based on Experimental Design.
Pharmaceutics
November 2024
College of Pharmacy and Research Institute for Drug Development, Pusan National University, 63 Busandaehak-ro, Geumjeong-gu, Busan 46241, Republic of Korea.
This study investigates the impact of supercritical antisolvent (SAS) process parameters on the particle formation of telmisartan, a poorly water-soluble drug. A fractional factorial design was employed to examine the influence of the SAS process parameters, including solvent ratio, drug solution concentration, temperature, pressure, injection rate of drug solution, and CO₂ flow rate, on particle formation. Solid-state characterizations of the SAS process particles using XRD and FT-IR confirmed their amorphous nature.
View Article and Find Full Text PDFSustainable Carbon Dots Loaded into Carboxymethylcellulose Based Hydrogels for Uterine Cancer Bioimaging.
Pharmaceutics
November 2024
iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, University of Lisboa, 1049-001 Lisbon, Portugal.
: The development of innovative materials for disease diagnostics and therapeutics is a fast-growing area of scientific research. In this work, we report the development of innovative hydrogels incorporating carbon dots (Cdots) for bioimaging purposes. : The Cdots were prepared using a sustainable and low-cost process, starting with an underused fiber from the Brazilian semiarid region.
View Article and Find Full Text PDFMechanical Properties and Degree of Conversion of a Novel 3D-Printing Model Resin.
Polymers (Basel)
December 2024
Glidewell Dental, Irvine, CA 92612, USA.
The aim of this study was to evaluate the mechanical properties and degree of conversion of a novel 3D-printing model resin and compare it to eight commercially available model resins. An experimental resin formulated by our proprietary resin technology along with DentaModel, NextDent 2, KeyModel Ultra, Rodin Model, Die and Model 2, DMR III, LCD Grey, and Grey Resin were used in this study. Parallelepiped specimens (2 × 2 × 25 mm, n = 5) were printed and measured for their flexural strength (FS), flexural modulus (FM), and modulus of resilience (MR) in accordance with ISO-4049.
View Article and Find Full Text PDFCharacterization and Evaluation of Zero-Order Release System Comprising Glycero-(9,10-trioxolane)-trialeate and PLA: Opportunity for Packaging and Biomedicine Applications.
Polymers (Basel)
December 2024
N.N. Semenov Federal Research Center for Chemical Physics Russian Academy of Sciences, 119991 Moscow, Russia.
Glycerol-(9,10-trioxolane) trioleate (OTOA) is a promising material that combines good plasticizing properties for PLA with profound antimicrobial activity, which makes it suitable for application in state-of-the-art biomedical and packaging materials with added functionality. On the other hand, application of OTOA in PLA-based antibacterial materials is hindered by a lack of knowledge on kinetics of the OTOA release. In this work, the release of glycero-(9,10-trioxolane) trioleate (OTOA) from PLA films with 50% OTOA content was studied during incubation in normal saline solution, and for the first time, the kinetics of OTOA release from PLA film was evaluated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!