Objective: To research the relationship between the seedling grade of Glycyrrhiza uralensis and the biomass accumulation, yield and quality of product, so as to provide basis for establishing seedling standard.

Methods: The weight of single seedling root was measured and the seedlings were divided into three grades by the clustering analysis. The different grade treatments of seedlings were made to conduct field trials and laboratory experiments.

Results: The weight of the whole plant and dry root in growth period of grades 1 and 2 (the weight of single root greater than 10.0 g) were larger than grade 3, and the yield was also the case. The root of Glycyrrhiza uralensis, growing for three years, of grades 1 and 2 contained higher contents of active compounds than grade 3 and the content of glycyrrhizin and liquiritin in the root of Glycyrrhiza uralensis of all treatments were higher than the standard of Chinese Pharmacopoeia (2010 edition).

Conclusion: The plant from the grades 1 and 2 seedlings with larger growth increment,higher output and better quality is the best seedling in cultivation.

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