Techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of ∼300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to ∼40 pm sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915380 | PMC |
| http://dx.doi.org/10.1038/nbt.3357 | DOI Listing |
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