AI Article Synopsis
- The study investigates the role of B-lymphocytes in chemical-induced asthma, focusing on how these cells respond after being sensitized to toluene diisocyanate (TDI).
- Recent findings show that transferring TDI-sensitized B cells into naïve or genetically modified mice can trigger an asthma-like response, highlighting their significance in asthma pathology.
- A proteomic analysis identified 16 key proteins, including cyclophilin A and cofilin 1, that are altered in sensitized B cells, suggesting they play a crucial role in initiating T lymphocyte-independent asthma responses.
Article Abstract
Introduction And Aim: The role of B-lymphocytes in chemical-induced asthma is largely unknown. Recent work demonstrated that transferring B lymphocytes from toluene diisocyanate (TDI)-sensitized mice into naïve mice, B cell KO mice and SCID mice, triggered an asthma-like response in these mice after a subsequent TDI-challenge. We applied two-dimensional difference gel electrophoresis (2D-DIGE) to describe the "sensitized signature" of B lymphocytes comparing TDI-sensitized mice with control mice.
Results: Sixteen proteins were identified that were significantly up- or down-regulated in B lymphocytes of sensitized mice. Particularly differences in the expression of cyclophilin A, cofilin 1 and zinc finger containing CCHC domain protein 11 could be correlated to the function of B lymphocytes as initiators of T lymphocyte independent asthma-like responses.
Conclusion: This study revealed important alterations in the proteome of sensitized B cells in a mouse model of chemical-induced asthma, which will have an important impact on the B cell function.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580316 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138791 | PLOS |
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