AI Article Synopsis
- A new photo-electrochemical sensor has been developed for detecting guanosine monophosphate (GMP) using a series of three enzyme reactions that ensure specificity and accurate measurement.
- The sensor works by converting GMP to GDP through guanylate kinase, then using pyruvate kinase and lactate dehydrogenase for subsequent conversions that lead to the electrochemical detection of lactate.
- The sensor is built using polyelectrolytes for immobilizing the enzymes and utilizes a CdS/ZnS quantum dot electrode that generates a photocurrent response when illuminated.
Article Abstract
A photo-electrochemical sensor for the specific detection of guanosine monophosphate (GMP) is demonstrated, based on three enzymes combined in a coupled reaction assay. The first reaction involves the adenosine triphosphate (ATP)-dependent conversion of GMP to guanosine diphosphate (GDP) by guanylate kinase, which warrants substrate specificity. The reaction products ADP and GDPare co-substrates for the enzymatic conversion of phosphoenolpyruvate to pyruvate in a second reaction mediated by pyruvate kinase. Pyruvate in turn is the co-substrate for lactate dehydrogenase that generates lactate via oxidation of nicotinamide adenine dinucleotide (reduced form) NADH to NAD(+). This third enzymatic reaction is electrochemically detected. For this purpose a CdS/ZnS quantum dot (QD) electrode is illuminated and the photocurrent response under fixed potential conditions is evaluated. The sequential enzyme reactions are first evaluated in solution. Subsequently, a sensor for GMP is constructed using polyelectrolytes for enzyme immobilization.
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| http://dx.doi.org/10.1002/smll.201501883 | DOI Listing |
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