Rationale: The bias associated with lipid contents in fish tissues is a recalcitrant topic for trophic studies using stable isotopes. Lipids are depleted in the heavy carbon isotope ((13)C) and the lipid content varies considerably among species, tissues and in both time and space. We have applied and assessed different correction methods for tropical tuna tissues.
Methods: We tested two types of normalization methods to deal with variable lipid content in liver, gonads, and white and red muscles of yellowfin, bigeye and skipjack tuna: a chemical extraction using dichloromethane and a mathematical correction based on three modeling approaches (linear, non-linear and mass balance models). We measured isotopic ratios of bulk and lipid-free tissues and assessed the predictive ability of the correction models with the lipid-free measurements. The parameters of the models were estimated from our dataset and from results from published studies on other species.
Results: Comparison between bulk, lipid-free and lipid-corrected isotopic ratios demonstrated that (1) chemical extraction using dichloromethane did not affect δ(15)N values; (2) the change in δ(13)C values after extraction was tissue-specific; (3) lipid-normalization models using published parameter estimates failed to predict lipid-corrected δ(13)C values; and (4) linear and non-linear models using parameters estimated for each tissue from our dataset provided accurate δ(13)C predictions for all tissues, and mass balance model for white muscle only.
Conclusions: Models using published estimates for parameters from other species cannot be used. Based on a range of lipid content that do not exceed 45%, we recommend the linear model to correct the bulk δ(13)C values in the investigated tissues but the parameters have to be estimated from a proportion of the original data for which chemical extraction is required and the isotopic values of bulk and lipid-free tissues are measured.
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http://dx.doi.org/10.1002/rcm.7215 | DOI Listing |
Int J Syst Evol Microbiol
January 2025
Department of Bio Health Science, Changwon National University, Changwon, Gyeongnam 51140, Republic of Korea.
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Cornell University, Department of Natural Resources and the Environment, Ithaca, NY.
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Biorefinery and Bioenergy Research Laboratory, Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, 201313, India.
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College of Life Science, Shenyang Normal University, Shenyang 110000, PR China.
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View Article and Find Full Text PDFInt J Syst Evol Microbiol
January 2025
Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
Three aerobic, pink-pigmented, Gram-negative, motile and rod-shaped bacterial strains, designated SD21, SI9 and SB2, were isolated from the phyllosphere of healthy litchis collected from three main producing sites of Guangdong Province, PR China. The 16S rRNA gene analysis showed that strains SD21 and SI9 belonged to the genus (.) with the highest similarity to DSM 19563 (98.
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