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Bacterial Shedu immune nucleases share a common enzymatic core regulated by diverse sensor domains.
Mol Cell
December 2024
Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA; Department of Molecular Biology, University of California, San Diego, La Jolla, CA, USA. Electronic address:
Prokaryotes possess diverse anti-bacteriophage immune systems, including the single-protein Shedu nuclease. Here, we reveal the structural basis for activation of Bacillus cereus Shedu. Two cryoelectron microscopy structures of Shedu show that it switches between inactive and active states through conformational changes affecting active-site architecture, which are controlled by the protein's N-terminal domain (NTD).
View Article and Find Full Text PDFA low-abundance class of Dicer-dependent siRNAs produced from a variety of features in .
Genome Res
December 2024
Department of Biology, Colorado State University, Fort Collins, Colorado 80523, USA;
Canonical small interfering RNAs (siRNAs) are processed from double-stranded RNA (dsRNA) by Dicer and associate with Argonautes to direct RNA silencing. In , 22G-RNAs and 26G-RNAs are often referred to as siRNAs but display distinct characteristics. For example, 22G-RNAs do not originate from dsRNA and do not depend on Dicer, whereas 26G-RNAs require Dicer but derive from an atypical RNA duplex and are produced exclusively antisense to their messenger RNA (mRNA) templates.
View Article and Find Full Text PDFComparison of proximal contact and contours of class II restoration in premolars and molars restored with three different matrix systems using FDI criteria: An study.
J Conserv Dent Endod
September 2024
Department of Conservative Dentistry and Endodontics, Y.M.T Dental College and Hospital, Kharghar, Navi Mumbai, India.
Article Synopsis
- The study examines the effectiveness of three different matrix systems—Tofflemire, Garrison, and Bioclear—used for Class II composite restorations in premolars and molars, focusing on their ability to create tight proximal contacts and proper contours.
- Using FDI criteria, the researchers conducted evaluations immediately after restoration placement, employing methods such as flossing and radiographic examination to assess the contact quality and marginal gaps.
- Statistical analysis indicated significant differences among the matrix systems in terms of the quality of proximal contact and contour, with results processed using SPSS and significance level set at p < 0.05.
Tuning Chemical DNA Ligation within DNA Crystals and Protein-DNA Cocrystals.
ACS Nanosci Au
October 2024
Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States.
Biomolecular crystals can serve as materials for a plethora of applications including precise guest entrapment. However, as grown, biomolecular crystals are fragile in solutions other than their growth conditions. For crystals to achieve their full potential as hosts for other molecules, crystals can be made stronger with bioconjugation.
View Article and Find Full Text PDFContext effects on repair of 5'-overhang DNA double-strand breaks induced by Cas12a in Arabidopsis.
Plant Direct
October 2024
Institut de Génétique, Reproduction et Développement, UMR CNRS 6293 - INSERM U1103 - Université Clermont Auvergne, Bât. CRBC, Faculté de Médecine Clermont-Ferrand Cedex 1 France.
Sequence-specific endonucleases have been key to the study of the mechanisms and control of DNA double-strand break (DSB) repair and recombination, and the availability of CRISPR-Cas nucleases over the last decade has driven rapid progress in the understanding and application of targeted recombination in many organisms, including plants. We present here an analysis of recombination at targeted chromosomal 5' overhang DSB generated by the FnCas12a endonuclease in the plant, . The much-studied Cas9 nuclease cleaves DNA to generate blunt-ended DSBs, but relatively less is known about the repair of other types of breaks, such as those with 5'-overhanging ends.
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