Dysentery is an inflammation of the intestine caused by the protozoan parasite Entamoeba histolytica and is a recurrent health problem affecting millions of people worldwide. Because of the magnitude of this disease, finding novel strategies for treatment that does not affect human cells is necessary. Ergosterol peroxide is a sterol particularly known as a major cytotoxic agent with a wide spectrum of biological activities produced by edible and medicinal mushrooms. The aim of this report is to evaluate the amoebicidal activity of ergosterol peroxide (5α, 8α-epidioxy-22E-ergosta-6,22-dien-3β-ol isolated from 5α, 8α-epidioxy-22E-ergosta-6,22-dien-3β-ol) (Jacq.) P. Kumm. f. sp. Florida. Our results show that ergosterol peroxide produced a strong cytotoxic effect against amoebic growth. The inhibitory concentration IC50 of ergosterol peroxide was evaluated. The interaction between E. histolytica and ergosterol peroxide in vitro resulted in strong amoebicidal activity (IC50 = 4.23 nM) that may be due to the oxidatory effect on the parasitic membrane. We also tested selective toxicity of ergosterol peroxide using a cell line CCL-241, a human epithelial cell line isolated from normal human fetal intestinal tissue. To the best of our knowledge, this is the first report on the cytotoxicity of ergosterol peroxide against E. histolytica, which uncovers a new biological property of the lipidic compound isolated from Pleurotus ostreatus (Jacq.) P. Kumm. f. sp. Florida.
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http://dx.doi.org/10.1002/ptr.5474 | DOI Listing |
Nat Prod Res
January 2025
Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, Hue City, Vietnam.
A new 14-membered cembrane- diterpenoid, jatrophainolide D (), along with six known compounds, ()-phytol (), lupeol (), stigmasterol (), ergosta-4,6,8 (14),22-tetraen-3-one (), stigmast-4-ene-3-one (), and ergosterol peroxide () were isolated from the aerial parts of . The structure of isolates was elucidated by IR, UV, 1D-/2D-NMR, and HRESI-TOFMS data as well as by comparison with the reported spectroscopic data. All isolates were evaluated the antibacterial activity against , , , , , and .
View Article and Find Full Text PDFBot Stud
January 2025
Institute of Fisheries Science, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Da'an Dist, Taipei, 106319, Taiwan (R.O.C.).
Background: Trichoderma species, known as biocontrol agents against plant diseases, contain diverse compounds, especially terpenoids, with various bioactivities. To facilitate the exploration of bioactive secondary metabolites of Trichoderma harzianum NTU2180, the OSMAC approach MS/MS molecular networking was applied in the current study.
Results: The feature-based molecular networking (FBMN) analysis showed that T.
Plants (Basel)
January 2025
Research Institute for Marine Traditional Chinese Medicine (Qingdao Academy of Chinese Medical Sciences), The SATCM's Key Unit of Discovering and Developing New Marine TCM Drugs, Key Laboratory of Marine Traditional Chinese Medicine in Shandong Universities, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
() is a necrotrophic fungus responsible for apple Valsa canker, which significantly diminishes apple production yields and quality in China. Our serendipitous findings revealed that genistein significantly inhibits the mycelial growth of , with an inhibition rate reaching 42.36 ± 3.
View Article and Find Full Text PDFWorld J Microbiol Biotechnol
December 2024
School of Chemical Engineering, Xiangtan University, Xiangtan, 411105, China.
Neofusicoccum parvum is one of the most hazardous pathogens causing mango fruit decay. The present study utilized trans-2-hexenal (TH), a typical antifungal component of plant essential oils (EOs), to control N. parvum both in vivo and in vitro, and attempted to explore the mechanisms involved.
View Article and Find Full Text PDFJ Appl Microbiol
December 2024
Centro de Excelencia en Productos y Procesos de Córdoba-CEPROCOR- Complejo Hospitalario, Santa María de Punilla, X6154 Córdoba, Argentina.
Aims: Thecaphora frezzii, the causal agent of peanut smut, causes significant grain losses in Argentina. Current control strategies are insufficient to manage this pathogen. We investigate the effect of antioxidants on the in vitro development of T.
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