The phage-shock protein (Psp) response is an extracytoplasmic response system that is vital for maintenance of the cytoplasmic membrane when the cell encounters stressful conditions. The paradigm of the Psp response has been established in Escherichia coli. The response has been shown to be important for survival during the stationary phase, maintenance of the proton motive force across membranes and implicated in virulence. In this study, we identified a putative PspA homologue in Burkholderia pseudomallei, annotated as BPSL2105. Similar to the induction of PspA in E. coli, the expression of B. pseudomallei BPSL2105 was induced by heat shock. Deletion of BPSL2105 resulted in a survival defect in the late stationary phase coincident with dramatic changes in the pH of the culture medium. The B. pseudomallei BPSL2105 deletion mutant also displayed reduced survival in macrophage infection - the first indication that the Psp response plays a role during intracellular pathogenesis in this species. The purified protein formed large oligomeric structures similar to those observed for the PspA protein of E. coli, and PspA homologues in Bacillus, cyanobacteria and higher plants, providing further evidence to support the identification of BPSL2105 as a PspA-like protein in B. pseudomallei.
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http://dx.doi.org/10.1099/mic.0.000175 | DOI Listing |
CPT Pharmacometrics Syst Pharmacol
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Department of Pharmacy, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Rheumatoid arthritis (RA) is a major public health concern, which can cause serious outcomes. Low-dose methotrexate (MTX) is a cornerstone in RA treatment, but there is significant heterogeneity in clinical response. To evaluate underlying sources of pharmacokinetic variability and clinical response of MTX, a physiologically based pharmacokinetic and pharmacodynamic (PBPK/PD) model was developed using PK-sim and Mobi (version 11.
View Article and Find Full Text PDFMicromachines (Basel)
November 2024
Department of Microsystem Technology, University of Applied Sciences Berlin, 12459 Berlin, Germany.
Shock wave boundary/layer interactions (SWBLIs) are critical in high-speed aerodynamic flows, particularly within supersonic regimes, where unsteady dynamics can induce structural fatigue and degrade vehicle performance. Conventional measurement techniques, such as pressure-sensitive paint (PSP), face limitations in frequency response, calibration complexity, and intrusive instrumentation. Similarly, MEMS-based sensors, like Kulite sensors, present challenges in terms of intrusiveness, cost, and integration complexity.
View Article and Find Full Text PDFNat Commun
January 2025
Biogen Inc, Cambridge, MA, USA.
Progressive supranuclear palsy (PSP) is a rare neurodegenerative disorder characterized by physical, cognitive, and behavioral impairments. The PSP Rating Scale (PSPRS) is a widely used and validated, clinical scale to monitor disease progression. Here we show the modification of PSPRS to improve clinical meaningfulness and sensitivity.
View Article and Find Full Text PDFPsychol Belg
January 2025
DivPsi -PSP (Portuguese Police -Clinical Psychology Department), Unidade Especial de Polícia -Quinta das Águas Livres, 2605-197 Belas, Portugal.
The clinical-organizational context (where clinical psychology services are provided in the individuals' professional setting) has still been insufficiently approached in research, namely the influence it may have on the response attitudes of individuals undergoing psychological assessment. Our main goal is to find out if, when psychological assessment occurs in the workplace context, patients being assessed present specific response bias that may have implications for the clinical results and correlative decisions. Five hundred and ten adult participants grouped in two samples of ambulatory patients - Clinical-Organizational Sample (COS = 238) and Clinical Sample (CS = 272) - were assessed with the Minnesota Multiphasic Personality Inventory-2-RF validity and substantive scales.
View Article and Find Full Text PDFInt Cancer Conf J
January 2025
Department of Allergy and Respiratory Medicine, Okayama University Hospital, Okayama, Japan.
Pulmonary sclerosing pneumocytoma (PSP) is a rare form of lung cancer that occasionally presents with lymph node and extrapulmonary metastases, and multiple lesions. The treatment of metastatic PSP remains undefined. This study reports the case of a 48-year-old female patient diagnosed with PSP following surgical intervention for a solitary nodule in the left lower lobe.
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