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Direct observation shows superposition and large scale flexibility within cytoplasmic dynein motors moving along microtubules. | LitMetric

Direct observation shows superposition and large scale flexibility within cytoplasmic dynein motors moving along microtubules.

Nat Commun

School of Molecular and Cellular Biology and Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.

Published: September 2015

AI Article Synopsis

  • Cytoplasmic dynein is a protein that moves along microtubules in cells, using ATP for energy, and is crucial for various cellular functions.* -
  • Researchers used cryo-electron microscopy to observe how dynein dimers from Dictyostelium discoideum interact with microtubules, noting their flexibility and different head positions.* -
  • The study reveals that dynein's structure and the arrangement of heads influence its movement direction and unique stepping patterns, providing insights into its functionality in cells.*

Article Abstract

Cytoplasmic dynein is a dimeric AAA(+) motor protein that performs critical roles in eukaryotic cells by moving along microtubules using ATP. Here using cryo-electron microscopy we directly observe the structure of Dictyostelium discoideum dynein dimers on microtubules at near-physiological ATP concentrations. They display remarkable flexibility at a hinge close to the microtubule binding domain (the stalkhead) producing a wide range of head positions. About half the molecules have the two heads separated from one another, with both leading and trailing motors attached to the microtubule. The other half have the two heads and stalks closely superposed in a front-to-back arrangement of the AAA(+) rings, suggesting specific contact between the heads. All stalks point towards the microtubule minus end. Mean stalk angles depend on the separation between their stalkheads, which allows estimation of inter-head tension. These findings provide a structural framework for understanding dynein's directionality and unusual stepping behaviour.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579568PMC
http://dx.doi.org/10.1038/ncomms9179DOI Listing

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